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Direct monitoring of equilibrium protein folding-unfolding by atomic force microscopy: pushing the limit

TitleDirect monitoring of equilibrium protein folding-unfolding by atomic force microscopy: pushing the limit
Publication TypeJournal Article
Year of Publication2019
AuthorsXiao, A, Li, H
JournalCHEMICAL COMMUNICATIONS
Volume55
Pagination12920-12923
Date PublishedNOV 7
ISSN1359-7345
Abstract

We report the direct observation of equilibrium folding-unfolding dynamics of a mechanically labile, three helix bundle protein GA using a commercial atomic force microscope (AFM). Our study extends the capability of commercial AFMs towards studying protein unfolding/refolding at equilibrium as well as increasingly complex biomolecular systems in the context of biological function.

DOI10.1039/c9cc06293a