|Title||Direct monitoring of equilibrium protein folding-unfolding by atomic force microscopy: pushing the limit|
|Publication Type||Journal Article|
|Year of Publication||2019|
|Authors||Xiao, A, Li, H|
|Date Published||NOV 7|
We report the direct observation of equilibrium folding-unfolding dynamics of a mechanically labile, three helix bundle protein GA using a commercial atomic force microscope (AFM). Our study extends the capability of commercial AFMs towards studying protein unfolding/refolding at equilibrium as well as increasingly complex biomolecular systems in the context of biological function.