|Title||Efficient chemoenzymatic synthesis of ADP-D-glycero-beta-D-manno-heptose and a mechanistic study of ADP-L-glycero-D-manno-heptose 6-epimerase|
|Publication Type||Journal Article|
|Year of Publication||2005|
|Authors||Read, JA, Ahmed, RA, Tanner, ME|
|Type of Article||Article|
|Keywords||biosynthesis, CARBON, D-MANNOHEPTOSE 6-EPIMERASE, EPIMERIZATION, PATHWAY|
[GRAPHICS] A chemoenzymatic synthesis of ADP-D-glycero-β-D-manno-heptose (ADP-D,D-Hep) is described in which D,D-Hep 7-phosphate is converted to ADP-D,D-Hep by two biosynthetic enzymes. This strategy allows access to the 6"-deuterated analogue, which upon incubation with the epimerase showed complete retention of the isotopic label at the 6"-position. This provides evidence for a direct oxidation mechanism in which the hydride initially transferred to the NADP(+) cofactor is subsequently returned to the same carbon in a nonstereospecific manner.
|URL||<Go to ISI>://000229574300042|