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Engineering Protein-Clay Nanosheets Composite Hydrogels with Designed Arginine-Rich Proteins

TitleEngineering Protein-Clay Nanosheets Composite Hydrogels with Designed Arginine-Rich Proteins
Publication TypeJournal Article
Year of Publication2019
AuthorsLv, S, Duan, T, Li, H
JournalLANGMUIR
Volume35
Pagination7255-7260
Date PublishedJUN 4
ISSN0743-7463
Abstract

Clay nanosheets (CNSs) have been widely used in the design of nanocomposite biomaterials. CNSs display a disk-like morphology with strong negatively charged surfaces. It has been shown that guanidinium-containing molecules can bind CNSs through noncovalent salt-bridge interactions and thus serve as ``molecular glues{''} for CNSs. Making use of the guanidinium side chain in arginine, here, we designed novel arginine-rich elastomeric proteins to engineer protein-CNS nanocomposite hydrogels. Our results showed that these arginine-rich proteins can interact with CNSs effectively and can cross-link CNSs into hydrogels. Rheological measurements showed that mechanical properties of the resultant hydrogels depended on the arginine content in the arginine-rich proteins as well as CNS/protein concentration. Compared with hydrogels constructed from CNSs or proteins alone, the novel protein-CNS nanocomposite hydrogels show much improved mechanical properties. Our work opens up a new avenue to engineer functional protein hydrogels for various applications.

DOI10.1021/acs.langmuir.9b00701