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INTERACTION OF PLASMA GELSOLIN WITH TROPOMYOSIN

TitleINTERACTION OF PLASMA GELSOLIN WITH TROPOMYOSIN
Publication TypeJournal Article
Year of Publication1992
AuthorsKoepf, EK, Burtnick, LD
JournalFebs Letters
Volume309
Pagination56-58
Date PublishedAug
Type of ArticleArticle
ISBN Number0014-5793
Keywordsactin, FILAMENTS, gelsolin, MUSCLE, PROTEIN, TROPOMYOSIN
Abstract

Horse plasma gelsolin labelled with benzophenone-4-isothiocyanate can be photochemically cross-linked to rabbit cardiac tropomyosin. The cross-linking proceeds with greater efficiency in calcium-containing buffers. Further evidence for interaction between these proteins is provided by retention of fluorescently labelled gelsolin on tropomyosin-agarose affinity columns and by the ability of tropomyosin to cause an increase in the fluorescence intensity of gelsolin labelled with fluorescein-5-isothiocyanate. Both of these effects require the presence of calcium ions.

URL<Go to ISI>://A1992JL06800013