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A new linear ion trap time-of-flight system with tandem mass spectrometry capabilities

TitleA new linear ion trap time-of-flight system with tandem mass spectrometry capabilities
Publication TypeJournal Article
Year of Publication1998
AuthorsCampbell, JM, Collings, BA, Douglas, DJ
JournalRapid Communications in Mass Spectrometry
Volume12
Pagination1463-1474
Type of ArticleArticle
ISBN Number0951-4198
KeywordsDECOMPOSITION, EJECTION, ELECTROSPRAY-IONIZATION, FREQUENCY STANDARD APPLICATIONS, MOLECULE REACTION, PARAMETERS, PERFORMANCE, PROTEINS, QUADRUPOLE, RESONANCE, SPECTROSCOPY
Abstract

A new hybrid linear ion trap time-of-flight mass spectrometer has been constructed and the first results for tandem-in-time MS/MS in a 2-dimensional Paul trap are presented. The instrument consists of an electrospray ionization source and two radio frequency (RF) only quadrupoles operating at a pressure of 7 mTorr N-2 which are coupled orthogonally to a linear TOFMS. Ions are trapped in an RF-only quadrupole through the application of timed stopping potentials on the entrance and exit apertures. Through the coupling of an auxiliary waveform generator on one pair of the ion trap rods a small dipolar excitation voltage is superimposed on the quadrupolar electric field. This voltage is used for precursor isolation via broadband excitation and collision induced dissociation through mass selective resonant excitation. Instrumental performance and choice of operating parameters are demonstrated by recording fragment spectra for +3 ions of renin substrate and fl ions of reserpine. TOFMS mass resolution (m/Delta m)(FWHM) is as high as 740 at m/z = 609. Fragmentation efficiency is greater than 50% and excitation mass resolution is 60-75. (C) 1998 John Wiley & Sons, Ltd.

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