Research & Teaching Faculty

Default Header Image

SPECIATION OF ARSENIC COMPOUNDS BY HPLC WITH HYDRIDE GENERATION ATOMIC-ABSORPTION SPECTROMETRY AND INDUCTIVELY-COUPLED PLASMA-MASS SPECTROMETRY DETECTION

TitleSPECIATION OF ARSENIC COMPOUNDS BY HPLC WITH HYDRIDE GENERATION ATOMIC-ABSORPTION SPECTROMETRY AND INDUCTIVELY-COUPLED PLASMA-MASS SPECTROMETRY DETECTION
Publication TypeJournal Article
Year of Publication1994
AuthorsLe, XC, Cullen, WR, Reimer, KJ
JournalTalanta
Volume41
Pagination495-502
Date PublishedApr
Type of ArticleArticle
ISBN Number0039-9140
KeywordsELEMENT-SPECIFIC DETECTOR, IDENTIFICATION, ION CHROMATOGRAPHY, MUSCLE, ORGANOARSENIC COMPOUNDS, PERFORMANCE LIQUID-CHROMATOGRAPHY, QUANTITATION, REFERENCE MATERIAL, SEPARATION, TRACE-ELEMENTS, WATER
Abstract

An arsenic specific detection system utilizing on-line microwave digestion and hydride generation atomic absorption spectrometry (MD/HGAAS) is described for arsenic speciation by using high performance liquid chromatography (HPLC). Both ion exchange chromatography and ion pair chromatography have been studied for the separation of arsenite, arsenate, monomethylarsonic acid (MMAA), dimethylarsinic acid (DMAA), and arsenobetaine (AB). When the commonly used mobile phases, phosphate and carbonate buffers at pH 7.5, are used on an anion exchange column, arsenite and AB co-elute. However, selective determination of these two arsenic compounds can be achieved by using the new detection system. Partial separation between arsenite and AB can be achieved by increasing the mobile phase pH to 10.3 and by using a polymer based anion exchange column. The detection limit obtained by using anion exchange chromatography with MD/HGAAS detection is approximately 10 ng/ml (or 200 pg for a 20-mul sample injection) for arsenite, DMAA and AB, 15 ng/ml (or 300 pg) for MMAA, and 20 ng/ml (or 400 pg) for arsenate. Complete separation of the five arsenic compounds is achieved on a reversed phase C18 column by using sodium heptanesulfonate as ion pair reagent. Comparable resolution between chromatographic peaks is obtained by using MD/HGAAS detection and inductively coupled plasma mass spectrometry (ICPMS) detection.

URL<Go to ISI>://A1994NJ81300003