|Title||Speciation of key arsenic metabolic intermediates in human urine|
|Publication Type||Journal Article|
|Year of Publication||2000|
|Authors||Le, XC, Lu, XF, Ma, MS, Cullen, WR, Aposhian, HV, Zheng, BS|
|Type of Article||Article|
|Keywords||ATOMIC FLUORESCENCE DETECTION, BLACKFOOT DISEASE, DRINKING-WATER, EXCRETION, EXPOSURE, INGESTION, METHYLATION, PERFORMANCE LIQUID-CHROMATOGRAPHY, SEPARATION, TEMPERATURE|
Biomethylation is the major human metabolic pathway for inorganic arsenic, and the speciation of arsenic metabolites is essential to a better understanding of arsenic metabolism and health effects. Here we describe a technique for the speciation of arsenic in human urine and demonstrate its application to the discovery of key arsenic metabolic intermediates, monomethylarsonous acid (MMA(III)) and dimethylarsinous acid (DMA(III)), in human urine. The study provides a direct evidence in support of the proposed arsenic methylation pathway in the human. The finding of MMA(III) and DMA(III) in human urine, along with recent studies showing the high toxicity of these arsenicals, suggests that the usual belief of arsenic detoxification by methylation needs to be reconsidered, The arsenic speciation technique is based on ion pair chromatographic separation of arsenic species on a 3-mum particle size column at 50 degreesC followed by hydride generation atomic fluorescence detection. Speciation of MMA(III), DMA(III), arsenite (As-III), arsenate (As-V), monomethylarsonic acid(MMA(V)), and dimethylarsinic acid (DMA(V)) in urine samples is complete in 6 min with detection limits of 0.5-2 mug/L. There is no need for any sample pretreatment. The capability of rapid analysis of trace levels of arsenic species, which resulted in the findings of the key metabolic intermediates, makes the technique useful for routine arsenic speciation analysis required for toxicological and epidemiological studies.
|URL||<Go to ISI>://000165094000012|