@article { ISI:A1992HV09000010,
	title = {REGION-DIRECTED MUTAGENESIS OF RESIDUES SURROUNDING THE ACTIVE-SITE NUCLEOPHILE IN BETA-GLUCOSIDASE FROM AGROBACTERIUM-FAECALIS},
	journal = {JOURNAL OF BIOLOGICAL CHEMISTRY},
	volume = {267},
	number = {15},
	year = {1992},
	month = {MAY 25},
	pages = {10248-10251},
	abstract = {The active site nucleophile of the beta-glucosidase of Agrobacterium faecalis has recently been identified by the use of inhibitors. A combination of site-directed and in vitro enzymatic mutagenesis was carried out on the beta-glucosidase to probe the structure of the active site region. Forty-three point mutations were generated at 22 different residues in the region surrounding the active site nucleophile, Glu358. Only five positions were identified which affected enzyme activity indicating that only a few key residues are important to enzyme activity, thus the enzyme can tolerate a number of single residue changes and still function. The importance of Glu358 to enzymatic function has been confirmed and other residues important to enzyme structure or function have been identified.},
	issn = {0021-9258},
	author = {TRIMBUR, DE and WARREN, RAJ and Withers, SG}
}
