@article { ISI:000074029400009,
	title = {Enzymatic synthesis of disaccharides using Agrobacterium sp. beta-glucosidase},
	journal = {CARBOHYDRATE RESEARCH},
	volume = {305},
	number = {3-4},
	year = {1997},
	month = {DEC},
	pages = {371-381},
	abstract = {The synthesis of several oligosaccharides, in yields up to 65\%, has been achieved by utilizing the transglycosylation activity of Agrobacterium sp. beta-glucosidase (EC 3.2.1.21). The regioselectivity of the glycosylation reaction was investigated using p-nitrophenyl beta-D-galactopyranoside, beta-D-mannopyranosyl fluoride and D-glucal as glycosyl donors and a series of phenyl 1-thio-, benzyl 1-thio-, and benzyl beta-D-glycopyranosides as glycosyl accepters. The isolated products were determined to contain a mixture of beta-(1 {\textendash}> 3) and/or beta-(1 {\textendash}> 4),glycosidic linkages for all glycosyl accepters, except for beta-D-galacto-configured accepters where a mixture of beta-(1 {\textendash}> 3) and beta-(1 {\textendash}> 6) linkages was observed. The wide range of glycosyl donors and accepters utilized by the enzyme in transglycosylation experiments demonstrates the versatility of this system for the aqueous-phase synthesis of oligosaccharides. (C) 1998 Elsevier Science Ltd.},
	issn = {0008-6215},
	doi = {10.1016/S0008-6215(97)10040-4},
	author = {Prade, H and MacKenzie, LF and Withers, SG}
}
