@article { ISI:000266794200003, title = {Recent Developments in Glycoside Synthesis with Glycosynthases and Thioglycoligases}, journal = {AUSTRALIAN JOURNAL OF CHEMISTRY}, volume = {62}, number = {6}, year = {2009}, note = {Symposium on Glycoscience, Chemistry and Chemical Biology, Oslo, NORWAY, 2007}, pages = {510-520}, abstract = {Glycosynthases are hydrolytically incompetent engineered glycosidases that catalyze the high-yielding synthesis of glycoconjugates from glycosyl fluoride donor substrates and appropriate acceptors. Glycosynthases from more than 10 glycoside hydrolase families have now been generated, allowing the synthesis of a wide range of oligosaccharides. Recent examples include glycosynthase-mediated syntheses of xylo-oligosaccharides, xyloglucans, glycolipids, and aryl glycosides. Glycosynthases have also now been generated from inverting glycosidases, increasing the range of enzyme scaffolds. Improvement of glycosynthase activity and broadening of specificity has been achieved through directed evolution approaches, and several novel high-throughput screens have been developed to allow this. Finally, metabolically stable glycoside analogues have been generated using another class of mutant glycosidases: thioglycoligases. Recent developments in all these aspects are discussed.}, issn = {0004-9425}, doi = {10.1071/CH09059}, author = {Rakic, Bojana and Withers, Stephen G.} }