@article { ISI:000302778000006,
	title = {Rice BGlu1 glycosynthase and wild type transglycosylation activities distinguished by cyclophellitol inhibition},
	journal = {CARBOHYDRATE RESEARCH},
	volume = {352},
	year = {2012},
	month = {MAY 1},
	pages = {51-59},
	abstract = {The rice BGlu1 beta-D-glucosidase nucleophile mutant E386G is a glycosynthase that catalyzes the synthesis of cellooligosaccharides from alpha-D-glucopyranosyl fluoride (GlcF) donor and p-nitrophenyl (pNP) cellobioside (Glc2-pNP) or cello-oligosaccharide acceptors. When activity with other donors and acceptors was tested, the initial enzyme preparation cleaved pNP-beta-D-glucopyranoside (Glc-pNP) and pNP-beta-D-fucopyranoside (Fuc-pNP) to pNP and glucose and fucose, suggesting contamination with wild type BGlu1 beta-glucosidase. The products from reaction of GlcF and Fuc-pNP included Fuc-beta-(1 -> 3)-Fuc-pNP, Glc-beta-(1 -> 3)Fuc-pNP, and Fuc-beta-(1 -> 4)-Glc-beta-(1 -> 3)-Fuc-pNP, suggesting the presence of both wild type BGlu1 and its glycosynthase. Inhibition of the BGlu1 beta-glucosidase activity within this preparation by cyclophellitol confirmed that the E386G glycosynthase preparation was contaminated with wild type BGlu1. Rice BGlu1 E386G-2, generated from a new construct designed to minimize back-mutation, showed glycosynthase activity without wild type hydrolytic or transglycosylation activity. E386G-2 catalyzed transfer of glycosyl residues from GlcF, alpha-L-arabinosyl fluoride, alpha-D-fucosyl fluoride, alpha-D-galactosyl fluoride, alpha-D-mannosyl fluoride, and alpha-D-xylosyl fluoride donors to Glc2-pNP acceptor. The synthetic products from the reactions of alpha-fucosyl fluoride and alpha-mannosyl fluoride donors were confirmed to result from addition of a beta-(1 -> 4)-linked glycosyl residue. Moreover, the E386G glycosynthase transferred glucose from GlcF donor to glucose, cellobiose, Glc-pNP, Fuc-pNP, pNP-beta-D-galactopyranoside, and pNP-beta-D-xylopyranoside acceptors, but little to pNP-beta-D-mannopyranoside. Production of longer oligosaccharides occurred most readily on acceptors with an equatorial 4-OH. Elimination of wild type contamination thereby allowed a clear assessment of BGlu1 E386G glycosynthase catalytic abilities. (C) 2012 Elsevier Ltd. All rights reserved.},
	issn = {0008-6215},
	doi = {10.1016/j.carres.2012.02.013},
	author = {Pengthaisong, Salila and Chen, Chi-Fan and Withers, Stephen G. and Kuaprasert, Buabarn and Cairns, James R. Ketudat}
}
