@article { ISI:000459222100003,
	title = {High-Throughput {\textquoteleft}{\textquoteleft}FP-Tag{{\textquoteright}{\textquoteright}} Assay for the Identification of Glycosyltransferase Inhibitors},
	journal = {JOURNAL OF THE AMERICAN CHEMICAL SOCIETY},
	volume = {141},
	number = {6},
	year = {2019},
	month = {FEB 13},
	pages = {2201-2204},
	abstract = {Bacterial capsular polysaccharides are important virulence factors. Capsular polysaccharides from several important Gram-negative pathogens share a conserved glycolipid terminus containing 3-deoxy-beta-D-manno-oct-2-ulosonic acid (beta-Kdo). The beta-Kdo glycosyltransferases responsible for synthesis of this conserved glycolipid belong to a new family of glycosyltransferases that shares little homology with other such enzymes, thereby representing an attractive antivirulence target. Here, we report the development of a fluorescence polarization-based, high-throughput screening assay (FP-tag) for beta-Kdo glycosyltransferases, and use it to identify a class of marine natural products as lead inhibitors. This {\textquoteleft}{\textquoteleft}FP-tag{{\textquoteright}{\textquoteright}} assay should be readily adaptable to high-throughput screens of other glycosyltransferases.},
	issn = {0002-7863},
	doi = {10.1021/jacs.8b10940},
	author = {Gao, Zhizeng and Ovchinnikova, Olga G. and Huang, Bo-Shun and Liu, Feng and Williams, David E. and Andersen, Raymond J. and Lowary, Todd L. and Whitfield, Chris and Withers, Stephen G.}
}
