@article {Leung:2019jo, title = {Electrodepositing DNA Self-Assembled Monolayers on Au: Detailing the Influence of Electrical Potential Perturbation and Surface Crystallography.}, journal = {ACS Sens.}, volume = {4}, number = {2}, year = {2019}, pages = {513{\textendash}520}, abstract = {

The preparation of DNA self-assembled monolayers (SAMs) on single-crystal gold bead electrodes using an applied potential is evaluated with in situ electrochemical fluorescence microscopy. Applying a constant deposition potential or a square-wave potential perturbation during the formation of DNA SAMs is compared for two different modification methods: DNA SAM formation on a clean gold surface followed by alkythiol backfilling (as is typically done in the literature) or via thiol-exchange on an alkylthiol-modified gold surface. DNA SAMs prepared from a chloride-containing deposition buffer were not significantly different when using either square-wave potential perturbation or at a constant applied potential even when considering different surface crystallographies. Greater variations were observed when applying more positive potentials for both DNA thiol-exchange and DNA adsorption on clean Au. Our results suggest that using either a constant potential or a square-wave potential perturbation for 5 min both create defects by weakening the gold-thiol interaction. When the deposition is performed with the adsorption of chloride ions from the electrolyte, the electrodeposition results in a similar increase in DNA coverage when compared to depositions performed at open circuit potentials.

}, doi = {10.1021/acssensors.8b01695}, url = {http://pubs.acs.org/doi/10.1021/acssensors.8b01695}, author = {Leung, Kaylyn K and Yu, Hua Zhong and Bizzotto, Dan} }