@article {doi:10.1002/elps.201900456,
	title = {Application of multisegment injection on quantification of creatinine and standard addition analysis of urinary 5-hydroxyindoleacetic acid simultaneously with creatinine normalization},
	journal = {ELECTROPHORESIS},
	volume = {41},
	number = {n/a},
	year = {2020},
	pages = {183-193},
	abstract = {<p>Abstract In this paper, the development of a simple dilute-and-shoot method for quantifying urinary creatinine by CE\&ndash;ESI\&ndash;MS was described. The creatinine analysis time was about 7\&nbsp;min/sample by conventional single injection (SI) method and can be significantly reduced to less than 2\&nbsp;min/sample with multi-segment injection (MSI). In addition, the standard addition analysis of 5-hydroxyindole-3-acetic acid (5-HIAA) and creatinine normalization was performed within one run by the MSI technique, and the total analysis time was 14-min faster compared to the SI method for analyzing the same set of samples. The uses of isotopic and non-isotopic internal standards (ISs) were compared. Creatinine-(methyl-13C) and 5-hydroxyindole-4,6,7-D3-3-acetic-D2 acid (5-HIAA-D5) used as isotopic ISs can provide both accurate and precise results. In contrast, 1,5,5-trimethylhydantoin (1,5,5-TH) used as the non-isotopic IS for creatinine may cause a bias of over 13\% in SI method and even worse when the MSI technique was used. Another compound, 2-methyl-3-indoleacetic acid (2-MIAA), was determined not suitable for MSI analysis of 5-HIAA due to endogenous interferences despite its acceptable performance in conventional methods of analysis.</p>},
	keywords = {capillary electrophoresis, creatinine normalization, MASS SPECTROMETRY, multisegment injection, urinary 5-hydroxyindoleacetic acid},
	doi = {10.1002/elps.201900456},
	url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/elps.201900456},
	author = {Huang, Zi-Ao and Scotland, Kymora B. and Li, Yueyang and Guo, Jian-Ping and McGeer, Patrick L. and Lange, Dirk and Chen, David D. Y.}
}
