@article {32713,
	title = {Rapid chemiexcitation of phenoxy-dioxetane luminophores yields ultrasensitive chemiluminescence assays},
	journal = {Chem. Sci.},
	volume = {10},
	year = {2019},
	pages = {1380-1385},
	abstract = {<p>The utility of dioxetane-based chemiluminescent probes in biosensing and bioimaging is being increasingly recognized. While phenoxy-dioxetane luminophores with fast chemiexcitation kinetics are highly desired{,} current luminophores suffer from slow chemiexcitation. Herein we describe a rational{,} computationally-supported design of phenoxy-dioxetanes with fast chemiexcitation kinetics. These new luminophores were designed to contain a substituent that promotes rapid chemiexcitation{,} emitting light up to 100-fold faster than currently known dioxetanes. We demonstrate the superiority of the new phenoxy-dioxetanes by preparing three chemiluminescent probes for NAD(P)H{,} which differ from each other in the rate of the chemiexcitation. Comparison of these probes reveals a correlation between the chemiexcitation rate and the probe sensitivity. We anticipate that these new phenoxy-dioxetanes could serve as an ideal platform for designing chemiluminescence probes with enhanced sensitivity for numerous bioassays.</p>},
	doi = {10.1039/C8SC04280B},
	url = {http://dx.doi.org/10.1039/C8SC04280B},
	author = {Hananya, Nir and Reid, Jolene P. and Green, Ori and Sigman, Matthew S. and Shabat, Doron}
}
