@article {4742,
	title = {Size exclusion chromatography does not require pores},
	journal = {Proceedings of the National Academy of Sciences of the United States of America},
	volume = {97},
	number = {13},
	year = {2000},
	note = {ISI Document Delivery No.: 327UHTimes Cited: 16Cited Reference Count: 24},
	month = {Jun},
	pages = {7064-7067},
	type = {Article},
	abstract = {Separation of macromolecules on the basis of their molecular weight by size exclusion chromatography has long been considered to be caused by the geometry-dependent partition of macromolecules between a continuous phase and the porous interior of a gel or cross-linked bead. The volume of a pore accessible to a solute is limited by its relative dimensions, so larger molecules will have access to a smaller volume and will remain in a bead for a shorter time than smaller solutes. Our recent alternate picture proposes that the partition coefficient can be calculated from a thermodynamic model for the free energy of mixing of the solute with the gel phase. Size-dependent exclusion caused by the unfavorable entropy of mixing associated with the partition is predicted; the magnitude of the effect is modified by enthalpic: interactions between the solute and the gel phase. This concept is extended here to describe the partition of macromolecules into a layer of terminally attached polymer chains grafted onto a solid bead. Both simple mean field and self-consistent field theory calculations predict size-dependent entropic exclusion, Experimental results obtained with neutral polymer chains grafted onto solid polystyrene latex beads confirm the predictions.},
	keywords = {CHAINS, MODEL},
	isbn = {0027-8424},
	url = {<Go to ISI>://000087811600027},
	author = {Brooks, D. E. and Haynes, C. A. and Hritcu, D. and Steels, B. M. and Muller, W.}
}
