@article {942,
	title = {Continuous electrophoretic purification of individual analytes from multicomponent mixtures},
	journal = {Analytical Chemistry},
	volume = {76},
	number = {8},
	year = {2004},
	note = {ISI Document Delivery No.: 816FWTimes Cited: 10Cited Reference Count: 36},
	month = {Apr},
	pages = {2298-2305},
	type = {Article},
	abstract = {Individual analytes can be isolated from multicomponent mixtures and collected in the outlet vial by carrying out electrophoretic purification through a capillary column. Desired analytes are allowed to migrate continuously through the column under the electric field while undesired analytes are confined to the inlet vial by application of a hydrodynamic counter pressure. Using pressure ramping and buffer replenishment techniques, 18\% of the total amount present in a bulk sample can be purified when the resolution to the adjacent peak is similar to3. With a higher resolution, the yield could be further improved. Additionally, by periodically introducing fresh buffer into the sample, changes in pH and conductivity can be mediated, allowing higher purity (99.5\%) to be preserved in the collected fractions. With an additional reversed cycle of flow counterbalanced capillary electrophoresis, any individual component in a sample mixture can be purified providing it can be separated in an electrophoresis system.},
	keywords = {CAPILLARY-ZONE-ELECTROPHORESIS, CHIRAL SEPARATIONS, CHROMATOGRAPHY, ENANTIOMERS, FRACTION COLLECTOR, FREE-FLOW ELECTROPHORESIS, ISOELECTRIC-FOCUSING SEPARATION, MASS-SPECTROMETRY, MEMBRANE, PREPARATIVE-SCALE},
	isbn = {0003-2700},
	url = {<Go to ISI>://000221096800019},
	author = {McLaren, D. G. and Chen, D. D. Y.}
}
