@article {3289, title = {ARSENIC BIOMETHYLATION BY THE MICROORGANISM APIOTRICHUM HUMICOLA IN THE PRESENCE OF L-METHIONINE-METHYL-D(3)}, journal = {Applied Organometallic Chemistry}, volume = {9}, number = {7}, year = {1995}, note = {ISI Document Delivery No.: TG189Times Cited: 15Cited Reference Count: 273rd International Conference on Environmental and Biological Aspects of Main Group Organometals (ICEBAMO 94)SEP, 1994BORDEAUX, FRANCESoc Francaise Chim, Royal Soc Chem, CNRS, EC Measurement \& Testing Program, French Minist Environm, French Minist Res \& Technol, Univ Bordeaux I, Univ Bordeaux II, IFREMER, INRA, Conseil Reg Aquitaine, Lyonnaise Eaux Dumez Water Co., Thermo Jarell Ash, John Wiley \& Sons, Freund Publ, Elsevier Sci Publ}, month = {Nov}, pages = {507-515}, type = {Proceedings Paper}, abstract = {The microorganism Apiotrichum humicola (previously known as Candida humicola) grown in the presence of either arsenate, arsenite, methyl-arsonic acid or dimethylarsinic acid, produces arsenic-containing metabolites in the growth medium. When L-methionine-methyl-d(3) is added to the cultures, the CD3 label is incorporated intact into the metabolites to a considerable extent to form deuterated dimethylarsenic and trimethylarsenic species, indicating that S-adenosylmethionine, or some related sulphonium compound, is involved in the biological methylation. Conclusive evidence of CD3 incorporation in the arsenicals found in the growth medium was provided by using a specially developed hydride generation-gas chromatography-mass spectrometry system (HG-GC-MS).}, keywords = {ARSENATE, ARSENITE, CHROMATOGRAPHY MASS SPECTROMETRY, DIMETHYLARSINIC ACID, EXTRACELLULAR, GAS, HYDRIDE GENERATION, HYDRIDE GENERATION GAS, L-METHIONINE-METHYL-D(3), methylarsonic acid, METHYLATION, MICROORGANISM, REDUCTION, S-ADENOSYLMETHIONINE, THIOLS, TRIMETHYLARSINE OXIDE}, isbn = {0268-2605}, url = {://A1995TG18900002}, author = {Cullen, W. R. and Li, H. and Pergantis, S. A. and Eigendorf, G. K. and Mosi, A. A.} }