@article {1612,
	title = {The structure of gelsolin bound to ATP},
	journal = {Journal of Molecular Biology},
	volume = {357},
	number = {3},
	year = {2006},
	note = {ISI Document Delivery No.: 030IYTimes Cited: 7Cited Reference Count: 28},
	month = {Mar},
	pages = {765-772},
	type = {Article},
	abstract = {Calcium activation of the actin-modifying properties of gelsolin is sensitive to ATP. Here, we show that soaking calcium-free gelsolin crystals in ATP-containing media results in ATP occupying a site that spans the two pseudosymmetrical halves of the protein. ATP binding involves numerous polar and hydrophobic contacts and is identical for the two copies of gelsolin related by non-crystallographic symmetry within the crystal. The gamma-phosphate of ATP participates in several charge-charge interactions consistent with the preference of gelsolin for ATP, as a binding partner, over ADP. In addition, disruption of the ATP-binding site through Ca2+ activation of gelsolin reveals why ATP binds more tightly to the inactive molecule, and suggests how the binding of ATP may modulate the sensitivity of gelsolin to calcium ions. Similarities between the ATP and PIP2 interactions with the C-terminal half of gelsolin are evident from their overlapping binding sites and in that both molecules bind more tightly in the absence of calcium ions. We propose a model for how PIP2 may bind to calcium-free gelsolin based on the ATP-binding site. (c) 2006 Elsevier Ltd. All rights reserved.},
	keywords = {5-BISPHOSPHATE, actin, ATP, BINDING-SITE, calcium, CRYSTAL-STRUCTURE, DOMAIN, gelsolin, HUMAN-PLASMA GELSOLIN, IDENTIFICATION, PHOSPHATIDYLINOSITOL 4, PIP2, PROTEIN, TERMINAL HALF},
	isbn = {0022-2836},
	url = {<Go to ISI>://000236629300007},
	author = {Urosev, D. and Ma, Q. and Tan, A. L. C. and Robinson, R. C. and Burtnick, L. D.}
}
@article {813,
	title = {Structure of the N-terminal half of gelsolin bound to actin: roles in severing, apoptosis and FAF},
	journal = {Embo Journal},
	volume = {23},
	number = {14},
	year = {2004},
	note = {ISI Document Delivery No.: 847NBTimes Cited: 41Cited Reference Count: 59},
	month = {Jul},
	pages = {2713-2722},
	type = {Article},
	abstract = {The actin filament-severing functionality of gelsolin resides in its N-terminal three domains (G1 - G3). We have determined the structure of this fragment in complex with an actin monomer. The structure reveals the dramatic domain rearrangements that activate G1 - G3, which include the replacement of interdomain interactions observed in the inactive, calcium-free protein by new contacts to actin, and by a novel G2 - G3 interface. Together, these conformational changes are critical for actin filament severing, and we suggest that their absence leads to the disease Finnish-type familial amyloidosis. Furthermore, we propose that association with actin drives the calcium-independent activation of isolated G1 G3 during apoptosis, and that a similar mechanism operates to activate native gelsolin at micromolar levels of calcium. This is the first structure of a filament-binding protein bound to actin and it sets stringent, high-resolution limitations on the arrangement of actin protomers within the filament.},
	keywords = {5-BISPHOSPHATE, actin, AMYLOIDOSIS-FINNISH TYPE, apoptosis, BINDING-SITE, CA2+ REGULATION, calcium, CRYSTAL-STRUCTURE, crystallographic structure, F-ACTIN, FAMILIAL AMYLOIDOSIS, FILAMENT BARBED ENDS, gelsolin, PHOSPHATIDYLINOSITOL 4, PLASMA GELSOLIN, X-RAY},
	isbn = {0261-4189},
	url = {<Go to ISI>://000223398800002},
	author = {Burtnick, L. D. and Urosev, D. and Irobi, E. and Narayan, K. and Robinson, R. C.}
}
@article {4487,
	title = {Unusual electrostatic effects on binding of C1q to anionic liposomes: Role of anionic phospholipid domains and their line tension},
	journal = {Biochemistry},
	volume = {38},
	number = {25},
	year = {1999},
	note = {ISI Document Delivery No.: 211AUTimes Cited: 19Cited Reference Count: 61},
	month = {Jun},
	pages = {8112-8123},
	type = {Article},
	abstract = {The binding of I-125-C1q to anionic liposomes was studied as a function of protein concentration, pH, ionic strength, and anionic lipid composition. The maximum amount of protein bound per micromole of lipid was very sensitive to electrostatic factors, increasing strongly with decreased pH and ionic strength or increased anionic Lipid content. The apparent association constant was independent of these electrostatic factors, however, in marked contrast to studies on basic peptide binding Co anionic lipid vesicles. Microscopic observations of large unilamellar Liposomes containing fluorescently labeled C1q or phosphatidylglycerol demonstrated, under conditions causing strong electrostatic interactions, that C1q and anionic lipids colocalized into domains whose radii of curvature were higher than that of the surrounding lipid. These domains were observed to bud and pinch off into brightly fluorescent vesicles. We propose a model for all of these observations in which the line tension or edge energy at the boundary of the domain resists its increase in circumference as the domain grows by electrostatic effects on binding, eventually resulting in vesiculation. We propose that under favorable electrostatic conditions, as larger domains form the edge energy balances the increases in the electrostatic contribution to binding, resulting in a net binding energy independent of electrostatic factors.},
	keywords = {5-BISPHOSPHATE, ACIDIC LIPIDS, ACTIVATES, BASIC PEPTIDES, COMPLEMENT, COMPLEMENT SUBCOMPONENT C1Q, HEPATIC-UPTAKE, LARGE UNILAMELLAR VESICLES, LATERAL DOMAINS, MEMBRANE DOMAINS, PHOSPHATIDYLINOSITOL 4, PROTEIN-KINASE-C},
	isbn = {0006-2960},
	url = {<Go to ISI>://000081138400028},
	author = {Bradley, A. J. and Maurer-Spurej, E. and Brooks, D. E. and Devine, D. V.}
}
