@article {4210,
	title = {Inhibition of liposome-induced complement activation by incorporated poly(ethylene glycol) lipids},
	journal = {Archives of Biochemistry and Biophysics},
	volume = {357},
	number = {2},
	year = {1998},
	note = {ISI Document Delivery No.: 119UCTimes Cited: 65Cited Reference Count: 50},
	month = {Sep},
	pages = {185-194},
	type = {Article},
	abstract = {Complement activation causes opsonization of foreign particles leading to particle elimination from the blood. Complement-mediated opsonization of charged and large liposomes presents a fundamental problem in their use to deliver therapeutic agents in vivo. To prolong the circulation half-lives of such liposomes, complement activation must be curtailed. The aim of this study was to assess the ability of poly(ethylene glycol)-lipids (PEG-lipids) to inhibit the in vitro activation of the classical pathway of complement in human serum by anionic liposomes. Incorporation of cholesterol-PEG(600) (CH-PEG(600)), cholesterol-PEG(1000) (CH-PEG(1000)), or phosphatidylethanolamine-PEG(2000) (PE-PEG(2000)) resulted in dose-dependent inhibition of Clq binding and complement activation. The dose of PEG-lipid at which complement activation was blocked was inversely related to the PEG chain length. Complement activation was strongly inhibited when 15 mole\% of CH-PEG(600), 10 mole\% CH-PEG(1000), or 5 mole\% PE-PEG(2000), was incorporated into 100-nm anionic liposomes. PEG-lipid incorporation into larger liposomes (240 nm) was also successful in blocking C1q binding and complement activation. Radiolabeled cholesterol-PEG(similar to 1400) was prepared and used to determine both the percentage of CH-PEG incorporated into the liposomes and the percentage maintained in the liposomes in the presence of 50\% human serum at 37 degrees C for up to 24 h. (C) 1998 Academic Press.},
	keywords = {C1q, CHOLESTEROL, CLEARANCE, complement activation, HEPATIC-UPTAKE, INVIVO, LARGE UNILAMELLAR VESICLES, LIPOSOMES, POLY(ETHYLENE GLYCOL), POLYETHYLENE-GLYCOL, PROLONGED CIRCULATION TIME, SERUM, SIZE, SURFACE-CHARGE},
	isbn = {0003-9861},
	url = {<Go to ISI>://000075915200002},
	author = {Bradley, A. J. and Devine, D. V. and Ansell, S. M. and Janzen, J. and Brooks, D. E.}
}
@inbook {2974,
	title = {PREPARATION AND ANALYSIS OF 2-PHASE SYSTEMS},
	booktitle = {Aqueous Two-Phase Systems},
	series = {Methods in Enzymology},
	volume = {228},
	year = {1994},
	note = {ISI Document Delivery No.: BA88LTimes Cited: 32Cited Reference Count: 20Review525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495},
	pages = {14-27},
	publisher = {Academic Press Inc},
	organization = {Academic Press Inc},
	address = {San Diego},
	keywords = {AQUEOUS 2-PHASE SYSTEMS, INTERFACIAL-TENSION, PARTITION, POLYETHYLENE-GLYCOL, POLYMER SYSTEMS, SALTS, SEPARATION},
	isbn = {0076-6879},
	url = {<Go to ISI>://A1994BA88L00002},
	author = {Brooks, D. E. and Norrisjones, R.}
}
@article {7142,
	title = {PARTITIONING IN AQUEOUS 2-PHASE SYSTEMS - RECENT RESULTS},
	journal = {Analytical Biochemistry},
	volume = {197},
	number = {1},
	year = {1991},
	note = {ISI Document Delivery No.: GB275Times Cited: 109Cited Reference Count: 139},
	month = {Aug},
	pages = {1-18},
	type = {Review},
	keywords = {2-POLYMER, AFFINITY PARTITION, COUNTERCURRENT DISTRIBUTION, LACTATE-DEHYDROGENASE, LIQUID LIQUID EXTRACTION, MATHEMATICAL-MODEL, PHASE-SEPARATION, POLYETHYLENE-GLYCOL, POLYMER SYSTEMS, SYSTEMS, THYLAKOID MEMBRANE},
	isbn = {0003-2697},
	url = {<Go to ISI>://A1991GB27500001},
	author = {Walter, H. and Johansson, G. and Brooks, D. E.}
}
