@article {2435,
	title = {A molecular barcoded yeast ORF library enables mode-of-action analysis of bioactive compounds},
	journal = {Nature Biotechnology},
	volume = {27},
	number = {4},
	year = {2009},
	note = {ISI Document Delivery No.: 430EOTimes Cited: 27Cited Reference Count: 60Ho, Cheuk Hei Magtanong, Leslie Barker, Sarah L. Gresham, David Nishimura, Shinichi Natarajan, Paramasivam Koh, Judice L. Y. Porter, Justin Gray, Christopher A. Andersen, Raymond J. Giaever, Guri Nislow, Corey Andrews, Brenda Botstein, David Graham, Todd R. Yoshida, Minoru Boone, Charles},
	month = {Apr},
	pages = {369-377},
	type = {Article},
	abstract = {We present a yeast chemical-genomics approach designed to identify genes that when mutated confer drug resistance, thereby providing insight about the modes of action of compounds. We developed a molecular barcoded yeast open reading frame (MoBY-ORF) library in which each gene, controlled by its native promoter and terminator, is cloned into a centromere-based vector along with two unique oligonucleotide barcodes. The MoBY-ORF resource has numerous genetic and chemical-genetic applications, but here we focus on cloning wild-type versions of mutant drug-resistance genes using a complementation strategy and on simultaneously assaying the fitness of all transformants with barcode microarrays. The complementation cloning was validated by mutation detection using whole-genome yeast tiling microarrays, which identified unique polymorphisms associated with a drug-resistant mutant. We used the MoBY-ORF library to identify the genetic basis of several drug-resistant mutants and in this analysis discovered a new class of sterol-binding compounds.},
	keywords = {1, 2, 3-BETA-D-GLUCAN SYNTHASE, CYCLOHEXIMIDE, DELETION MUTANTS, ELONGATION-FACTOR, GENE-DELETION, MAMMALIAN PROTEIN, ORDERED ARRAYS, OVEREXPRESSION STRAINS, resistance, SACCHAROMYCES-CEREVISIAE GENOME, SPONGE THEONELLA SP},
	isbn = {1087-0156},
	url = {<Go to ISI>://000264971800023},
	author = {Ho, C. H. and Magtanong, L. and Barker, S. L. and Gresham, D. and Nishimura, S. and Natarajan, P. and Koh, J. L. Y. and Porter, J. and Gray, C. A. and Andersen, R. J. and Giaever, G. and Nislow, C. and Andrews, B. and Botstein, D. and Graham, T. R. and Yoshida, M. and Boone, C.}
}
@article {1518,
	title = {Exploring the mode-of-action of bioactive compounds by chemical-genetic profiling in yeast},
	journal = {Cell},
	volume = {126},
	number = {3},
	year = {2006},
	note = {ISI Document Delivery No.: 075KITimes Cited: 111Cited Reference Count: 68Parsons, Ainslie B. Lopez, Andres Givoni, Inmar E. Williams, David E. Gray, Christopher A. Porter, Justin Chua, Gordon Sopko, Richelle Brost, Renee L. Ho, Cheuk-Hei Wang, Jiyi Ketela, Troy Brenner, Charles Brill, Julie A. Fernandez, G. Esteban Lorenz, Todd C. Payne, Grego S. Ishihara, Satoru Ohya, Yoshikazu Andrews, Brenda Hughes, Timothy R. Frey, Brendan J. Graham, Todd R. Andersen, Raymond J. Boone, Charles},
	month = {Aug},
	pages = {611-625},
	type = {Article},
	abstract = {Discovering target and off-target effects of specific compounds is critical to drug discovery and development. We generated a compendium of "chemical-genetic interaction" profiles by testing the collection of viable yeast haploid deletion mutants for hypersensitivity to 82 compounds and natural product extracts. To cluster compounds with a similar mode-of-action and to reveal insights into the cellular pathways and proteins affected, we applied both a hierarchical clustering and a factorgram method, which allows a gene or compound to be associated with more than one group. In particular, tamoxifen, a breast cancer therapeutic, was found to disrupt calcium homeostasis and phosphatidylserine (PS) was recognized as a target for papuamide B, a cytotoxic lipopeptide with anti-HIV activity. Further, the profile of crude extracts resembled that of its constituent purified natural product, enabling detailed classification of extract activity prior to purification. This compendium should serve as a valuable key for interpreting cellular effects of novel compounds with similar activities.},
	keywords = {1, 3-BETA-D-GLUCAN SYNTHASE, BREAST-CANCER CELLS, DELETION MUTANTS, drug, EXPRESSION, GENOME-WIDE ANALYSIS, HAPLOINSUFFICIENCY, INDUCED, SACCHAROMYCES-CEREVISIAE, THEONELLA-SWINHOEI, TRANSCRIPTION FACTOR},
	isbn = {0092-8674},
	url = {<Go to ISI>://000239883400019},
	author = {Parsons, A. B. and Lopez, A. and Givoni, I. E. and Williams, D. E. and Gray, C. A. and Porter, J. and Chua, G. and Sopko, R. and Brost, R. L. and Ho, C. H. and Wang, J. Y. and Ketela, T. and Brenner, C. and Brill, J. A. and Fernandez, G. E. and Lorenz, T. C. and Payne, G. S. and Ishihara, S. and Ohya, Y. and Andrews, B. and Hughes, T. R. and Frey, B. J. and Graham, T. R. and Andersen, R. J. and Boone, C.}
}
