@article {1518,
	title = {Exploring the mode-of-action of bioactive compounds by chemical-genetic profiling in yeast},
	journal = {Cell},
	volume = {126},
	number = {3},
	year = {2006},
	note = {ISI Document Delivery No.: 075KITimes Cited: 111Cited Reference Count: 68Parsons, Ainslie B. Lopez, Andres Givoni, Inmar E. Williams, David E. Gray, Christopher A. Porter, Justin Chua, Gordon Sopko, Richelle Brost, Renee L. Ho, Cheuk-Hei Wang, Jiyi Ketela, Troy Brenner, Charles Brill, Julie A. Fernandez, G. Esteban Lorenz, Todd C. Payne, Grego S. Ishihara, Satoru Ohya, Yoshikazu Andrews, Brenda Hughes, Timothy R. Frey, Brendan J. Graham, Todd R. Andersen, Raymond J. Boone, Charles},
	month = {Aug},
	pages = {611-625},
	type = {Article},
	abstract = {Discovering target and off-target effects of specific compounds is critical to drug discovery and development. We generated a compendium of "chemical-genetic interaction" profiles by testing the collection of viable yeast haploid deletion mutants for hypersensitivity to 82 compounds and natural product extracts. To cluster compounds with a similar mode-of-action and to reveal insights into the cellular pathways and proteins affected, we applied both a hierarchical clustering and a factorgram method, which allows a gene or compound to be associated with more than one group. In particular, tamoxifen, a breast cancer therapeutic, was found to disrupt calcium homeostasis and phosphatidylserine (PS) was recognized as a target for papuamide B, a cytotoxic lipopeptide with anti-HIV activity. Further, the profile of crude extracts resembled that of its constituent purified natural product, enabling detailed classification of extract activity prior to purification. This compendium should serve as a valuable key for interpreting cellular effects of novel compounds with similar activities.},
	keywords = {1, 3-BETA-D-GLUCAN SYNTHASE, BREAST-CANCER CELLS, DELETION MUTANTS, drug, EXPRESSION, GENOME-WIDE ANALYSIS, HAPLOINSUFFICIENCY, INDUCED, SACCHAROMYCES-CEREVISIAE, THEONELLA-SWINHOEI, TRANSCRIPTION FACTOR},
	isbn = {0092-8674},
	url = {<Go to ISI>://000239883400019},
	author = {Parsons, A. B. and Lopez, A. and Givoni, I. E. and Williams, D. E. and Gray, C. A. and Porter, J. and Chua, G. and Sopko, R. and Brost, R. L. and Ho, C. H. and Wang, J. Y. and Ketela, T. and Brenner, C. and Brill, J. A. and Fernandez, G. E. and Lorenz, T. C. and Payne, G. S. and Ishihara, S. and Ohya, Y. and Andrews, B. and Hughes, T. R. and Frey, B. J. and Graham, T. R. and Andersen, R. J. and Boone, C.}
}
@article {574,
	title = {Complementary on-line preconcentration strategies for steroids by capillary electrophoresis},
	journal = {Journal of Chromatography A},
	volume = {1013},
	number = {1-2},
	year = {2003},
	note = {ISI Document Delivery No.: 727KJTimes Cited: 47Cited Reference Count: 4416th International Symposium on Microscale Separation and AnalysisJAN 17-22, 2003SAN DIEGO, CALIFORNIA},
	month = {Sep},
	pages = {65-76},
	type = {Proceedings Paper},
	abstract = {Complementary on-line preconcentration strategies are needed when analyzing different classes of solutes in real samples by capillary electrophoresis (CE) with UV detection. The performance of three different on-line preconcentration (focusing) techniques under alkaline conditions was examined in terms of their selectivity and sensitivity enhancement for a group of steroids, including classes of androgens, corticosteroids and estrogens. Electrokinetic focusing of large sample injection plugs (up to 28\% of effective capillary length or 22.1 cm) directly on-capillary can be tuned for specific classes of steroids based on changes in their mobility (velocity) using a multi-section electrolyte system in CE. A dynamic pH junction was applied for the selective resolution and focusing of weakly acidic estrogens using borate, pH 11.0 and pH 8.0 in the background electrolyte and the sample, respectively. Sweeping, using an anionic bile acid surfactant and neutral gamma-cyclodextrin (gamma-CD) under alkaline conditions (pH 8), resulted in focusing and separation of the moderately hydrophobic (non-ionic) classes of steroids, such as androgen and corticosteroids. Optimal focusing and resolution of all test steroids under a single buffer condition was realized by a dynamic pH junction-sweeping format using borate, pH 11.0 and bile acid surfactant with gamma-CD in the BGE, whereas the sample is devoid of surfactant at pH 8.0. The design of selective on-line focusing strategies in CE is highlighted by the analysis of microgram amounts of ethynyl estradiol derived from a female contraceptive pill extract using the dynamic pH junction method, which resulted in over a 100-fold enhancement in concentration sensitivity. (C) 2003 Elsevier B.V. All rights reserved.},
	keywords = {dynamic pH, dynamic pH junction, EPINEPHRINE, FLUORESCENCE DETECTION, INDUCED, INJECTION, MICELLAR ELECTROKINETIC CHROMATOGRAPHY, NEUTRAL ANALYTES, sample preparation, SAMPLE SELF-STACKING, SEPARATION, steroids, sweeping, TAURODEOXYCHOLATE, ZONE-ELECTROPHORESIS},
	isbn = {0021-9673},
	url = {<Go to ISI>://000185656900009},
	author = {Britz-McKibbin, P. and Ichihashi, T. and Tsubota, K. and Chen, D. D. Y. and Terabe, S.}
}
@article {498,
	title = {An atmospheric pressure ion lens that improves nebulizer assisted electrospray ion sources},
	journal = {Journal of the American Society for Mass Spectrometry},
	volume = {13},
	number = {8},
	year = {2002},
	note = {ISI Document Delivery No.: 588METimes Cited: 7Cited Reference Count: 27},
	month = {Aug},
	pages = {906-913},
	type = {Article},
	abstract = {An atmospheric pressure ion lens improves the performance and ease of use of a nebulizer assisted electrospray (ion spray) ion source. The lens is comprised of an oblong-shaped stainless steel ring attached to an external high voltage power supply. The lens is located near the tip of the conductive sprayer, and is maintained at a potential less than that of the sprayer. The ion lens improves the shape of the equipotential lines in the vicinity of the sprayer tip. This lens gives approximately a 2-fold reduction in the signal RSD, a 2-fold increase in the ion signal, an increase in the number of multiply charged ions, and a much broader range of usable sprayer positions. (C) 2002 American Society for Mass Spectrometry.},
	keywords = {CAPILLARY-ZONE-ELECTROPHORESIS, DISSOCIATION, EVAPORATION, IDENTIFICATION, INDUCED, INTERFACE, IONIZATION MASS-SPECTROMETRY, JUNCTION, MECHANISM, PROTEIN-ANALYSIS, SHEATHLESS},
	isbn = {1044-0305},
	url = {<Go to ISI>://000177704600002},
	author = {Schneider, B. B. and Douglas, D. J. and Chen, D. D. Y.}
}
@article {5203,
	title = {Adsorption of DOPC onto Hg from the G|S interface and from a liposomal suspension},
	journal = {Journal of Electroanalytical Chemistry},
	volume = {516},
	number = {1-2},
	year = {2001},
	note = {ISI Document Delivery No.: 507LLTimes Cited: 21Cited Reference Count: 53},
	month = {Dec},
	pages = {73-82},
	type = {Article},
	abstract = {The adsorption of various physical forms of dioleoyl phosphatidylcholine (DOPC) onto a Hg electrode have been studied. The three systems investigated were adsorption of DOPC from the monolayer state present on the gas \ solution (G \ S) interface, the adsorption of 100 nm DOPC liposomes present in the electrolyte, and a mixed system consisting of the co-adsorption of the monolayer and liposomes. The adsorption was characterized by capacitance measurements, chronoamperometry and impedance spectroscopy. The adsorbed layer produced by liposome adsorption was distinctly different as compared to the layer adsorbed from the G \ S interface, whereas the co-adsorbed layer retained most of the liposome layer qualities. A low capacitance region, centered at - 0.40 V (SCE) with values of 1.85, and 1.45 muF cm(-2) for the G \ S and the liposome system were, respectively observed. This region was stable with respect to very negative potential cycling only for the liposome systems. Three phase transitions (denoted by pseudo-capacitance peaks) were noted for the three systems under study; two corresponding to a change in the adsorbed state, and one corresponding to the adsorption-desorption process. The kinetics of these changes were quite different and depended on the nature of the adsorbing species. The most significant differences were observed when measuring the capacity over a large range of perturbation frequency. At low frequencies, desorption of the DOPC layer was observed at very negative potentials for all the systems studied. As the perturbation frequency was increased, the capacitance measured for the G \ S system maintained this characteristic, but surprisingly this was not the case for the liposome systems. In both cases (liposome and co-adsorbed) the capacity, calculated assuming a series RC circuit, was found to be significantly lower than the capacity measured for the Hg \ 0.1 M KCl interface. Preliminary impedance spectra are presented illustrating the non-ideal behavior of the liposomal system. This phenomenon is undergoing further study. A possible mechanism for the adsorption of DOPC from the liposomal state is also presented. (C) 2001 Elsevier Science B.V. All rights reserved.},
	keywords = {AGGREGATION PROCESSES, CAPACITANCE, CYTOCHROME-C-OXIDASE, ELECTRIFIED INTERFACES, GRAMICIDIN CHANNEL FUNCTION, INDUCED, lipid monolayer, liposome adsorption, mercury, MERCURY-ELECTROLYTE INTERFACE, phase change kinetics, PHASE-TRANSITIONS, PHOSPHOLIPID MONOLAYERS, SODIUM DODECYL-SULFATE, STRUCTURAL-CHANGES, SUPPORTED LIPID BILAYERS},
	isbn = {0022-0728},
	url = {<Go to ISI>://000173029900010},
	author = {Stauffer, V. and Stoodley, R. and Agak, J. O. and Bizzotto, D.}
}
@article {3777,
	title = {A biomimetic study of cytochrome P450 related oxidations of toluenes using synthetic hemin},
	journal = {Bulletin of the Chemical Society of Japan},
	volume = {69},
	number = {12},
	year = {1996},
	note = {ISI Document Delivery No.: WB844Times Cited: 6Cited Reference Count: 68},
	month = {Dec},
	pages = {3513-3521},
	type = {Article},
	abstract = {A mechanistic study of the oxidation of toluene and its derivatives in a model system designed to mimic cytochrome P450 (P450) using iron tetrakis(2,6-dichlorophenyl)porphyrin chloride (FeTPPC8Cl) and pentafluoroiodosylbenzene (PFIB:C(6)F(5)lO) in dichloromethane is reported. The oxidation products were the corresponding benzyl alcohols and aldehydes; no further oxidation products were observed. The relative reaction rates were obtained in competitive reactions including the use of deuterated analogues. In the present model system, Hammett plots and isotope effects indicated conclusively that the rate-determining step was a hydrogen atom abstraction to the oxoiron intermediate which was then followed by a rebound of an OH group. The concerted mechanism and the rebound mechanism were discussed in terms of the differences in reactivities between the model system and natural P450 enzymes and similar monooxygenation enzymes.},
	keywords = {ALIPHATIC, AROMATIC-COMPOUNDS, CRYSTAL-STRUCTURE, DEPENDENT MONO-OXYGENASES, HYDROGEN-PEROXIDE, HYDROXYLATION, INDUCED, IRON(III) PORPHYRINS, LIVER MICROSOMAL CYTOCHROME-P-450, METABOLISM, MODEL SYSTEMS, RAT-LIVER},
	isbn = {0009-2673},
	url = {<Go to ISI>://A1996WB84400017},
	author = {Nakano, T. and Agatsuma, N. and Kodama, S. and Kakuda, H. and Dolphin, D.}
}
