@article {444,
	title = {Carbohydrate-binding modules recognize fine substructures of cellulose},
	journal = {Journal of Biological Chemistry},
	volume = {277},
	number = {52},
	year = {2002},
	note = {ISI Document Delivery No.: 631QDTimes Cited: 20Cited Reference Count: 36},
	month = {Dec},
	pages = {50245-50254},
	type = {Article},
	abstract = {Competition isotherms are used to identify the set of cellulose substructures to which cellulose binding modules (CBMs) from families 2a, 3, 4, 9, and 17 bind. The experiments are based on coupling a unique fluorescent tag to each CBM in a manner that does not alter the natural binding properties of the CBM and therefore allows the surface and solution concentrations of each CBM to be monitored as a function of time and composition. Adsorption and surface exchange of like or competing CBMs are monitored using a range of cellulose preparations varying in both crystallinity and provenance. CBMs from families 2a, 3,4,9, and 17 are shown to recognize different physical forms of prepared cellulose. The demonstration of the very fine binding specificity of cellulose-specific CBMs implies that the polysaccharide targets of CBMs extend down to the resolution of cellulose microstructures.},
	keywords = {BACTERIAL, CELLULASE, CRYSTALLINE CELLULOSE, DOMAIN, EXOGLUCANASE, FIMI CENC, NMR-SPECTROSCOPY, PROTEIN-A, SPECIFICITY, TRYPTOPHAN RESIDUES, XYLANASE 10A},
	isbn = {0021-9258},
	url = {<Go to ISI>://000180177700007},
	author = {McLean, B. W. and Boraston, A. B. and Brouwer, D. and Sanaie, N. and Fyfe, C. A. and Warren, R. A. J. and Kilburn, D. G. and Haynes, C. A.}
}
@article {3744,
	title = {Microbial degradation of pyrene and characterization of a metabolite},
	journal = {Science of the Total Environment},
	volume = {177},
	year = {1996},
	note = {ISI Document Delivery No.: TP870Times Cited: 10Cited Reference Count: 37},
	month = {Jan},
	pages = {17-29},
	type = {Article},
	abstract = {We report the degradation of pyrene by microorganisms present in sea water and sediment samples that were obtained from Kitimat Arm, BC, Canada, an environment that is highly contaminated by polycyclic aromatic hydrocarbons because of nearby aluminum smelting activity. The utilization of pyrene as sole carbon source by the microorganisms is demonstrated by monitoring the disappearance of pyrene and the production of its metabolite in the cultures. Pyrene (10 mu g) is degraded to an undetectable level after 120 h of incubation with 0.5 mi of the enriched culture and 10 ml of mineral salt medium. The extent of mineralization is also assessed by determining the radioactivity of the (CO2)-C-14 produced from the cultures containing C-14-labeled pyrene: up to 65\% of the pyrene is mineralized after 12 days of incubation. A metabolite that accumulates in the pyrene degradation cultures can be isolated. This metabolite is identified as cis-dihydrodiol pyrene, an initial ring oxidation product, on the basis of its chromatographic behavior, absorption spectrum, fluorescence excitation and emission spectra, and mass spectra.},
	keywords = {BACTERIAL, BIODEGRADATION, bioremediation, cis-dihydrodiol pyrene, CONTAMINATED SOIL, HYDROCARBONS, METABOLITE, microbial degradation, MINERALIZATION, MYCOBACTERIUM SP, NAPHTHALENE, OXIDATION, PHENANTHRENE, polycyclic aromatic, POLYCYCLIC AROMATIC-HYDROCARBONS, PYRENE, SEDIMENT},
	isbn = {0048-9697},
	url = {<Go to ISI>://A1996TP87000003},
	author = {Li, X. F. and Cullen, W. R. and Reimer, K. J. and Le, X. C.}
}
