@article {4916,
	title = {Cell-based screen for antimitotic agents and identification of analogues of rhizoxin, eleutherobin, and paclitaxel in natural extracts},
	journal = {Cancer Research},
	volume = {60},
	number = {18},
	year = {2000},
	note = {ISI Document Delivery No.: 358GQTimes Cited: 62Cited Reference Count: 55},
	month = {Sep},
	pages = {5052-5058},
	type = {Article},
	abstract = {We describe a cell-based assay for antimitotic compounds that is suitable for drug discovery and for quantitative determination of antimitotic activity, In the assay, cells arrested in mitosis as a result of exposure to antimitotic agents in pure form or in crude natural extracts are detected by ELISA using the monoclonal antibody TG-3, The assay was used to screen >24,000 extracts of marine microorganisms and invertebrates and terrestrial plants and to guide the purification of active compounds from 5 of 119 positive extracts. A new rhizoxin analogue was found in a Pseudomonas species, six new eleutherobin analogues were identified from the octocoral Erythropodium caribaeorum, and two paclitaxel analogues were found in the stem bark of the tree Ilex macrophylla. The assay was also used for quantitative comparison of the antimitotic activity of different analogues. It revealed the importance of the C-11 to C-13 segment of the diterpene core of eleutherobin for its antimitotic activity. The identification of antimitotic compounds in very low abundance and their high (0.5\%) occurrence in natural extracts indicates that drug discovery efforts using this cell-based assay may lead to the identification of structurally novel antimitotic agents.},
	keywords = {AGENT, ANTITUMOR, BINDING, fungus, INHIBITION, MACROCYCLIC LACTONE ANTIBIOTICS, MICROTUBULE-STABILIZING AGENTS, MITOTIC SPINDLES, RHIZOPUS-CHINENSIS, TAXOL, TUBULIN POLYMERIZATION},
	isbn = {0008-5472},
	url = {<Go to ISI>://000089550600013},
	author = {Roberge, M. and Cinel, B. and Anderson, H. J. and Lim, L. and Jiang, X. X. and Xu, L. and Bigg, C. M. and Kelly, M. T. and Andersen, R. J.}
}
@article {4774,
	title = {Solid-state and solution conformations of eleutherobin obtained from X-ray diffraction analysis and solution NOE data},
	journal = {Tetrahedron Letters},
	volume = {41},
	number = {16},
	year = {2000},
	note = {ISI Document Delivery No.: 307YFTimes Cited: 2Cited Reference Count: 13},
	month = {Apr},
	pages = {2811-2815},
	type = {Article},
	abstract = {Single crystal X-ray diffraction analysis has revealed the solid-state conformation of the microtubule-stabilizing diterpenoid eleutherobin (1). NOE data obtained for 1 in CDCl3 and DMSO-d(6) are consistent with solution conformations that are virtually identical to the solid state conformation. (C) 2000 Elsevier Science Ltd. All rights reserved.},
	keywords = {MICROTUBULE-STABILIZING AGENTS, PACLITAXEL, TAXOL},
	isbn = {0040-4039},
	url = {<Go to ISI>://000086681200012},
	author = {Cinel, B. and Patrick, B. O. and Roberge, M. and Andersen, R. J.}
}
