@article {4194,
	title = {Granulatimide and isogranulatimide, aromatic alkaloids with G2 checkpoint inhibition activity isolated from the Brazilian ascidian Didemnum granulatum: Structure elucidation and synthesis},
	journal = {Journal of Organic Chemistry},
	volume = {63},
	number = {26},
	year = {1998},
	note = {ISI Document Delivery No.: 153LPTimes Cited: 72Cited Reference Count: 34},
	month = {Dec},
	pages = {9850-9856},
	type = {Article},
	abstract = {Crude methanol ex-tracts of the ascidian Didemum granulatum collected in Brazil showed activity in a new screen for G2 cell cycle checkpoint inhibitors. Bioassay-guided fractionation of the extract yielded the known alkaloids didemnimides A (1) and D (2), the new alkaloid didemnimide E (3), and a new G2 checkpoint inhibitor. Two candidate structures for the inhibitor, named granulatimide (4) and isogranulatimide (5), have been prepared via a short and efficient biomimetic synthesis involving the photolysis of didemnimide A (1). The synthesis revealed that the correct structure for the naturally occurring G2 checkpoint inhibitor is isogranulatimide (5). Granulatimide (4), the other candidate structure, was also found to be a G2 checkpoint inhibitor, and it was subsequently detected in chromatographic fractions associated with purification of D. granulatum alkaloids. Granulatimide (4) and isogranulatimide (5) represent the first examples of a new class of G2 specific cell cycle checkpoint inhibitors and the first ones identified through a rational screening program.},
	keywords = {BHK CELLS, CAFFEINE, CELL-CYCLE CHECKPOINTS, DNA-REPLICATION, HELA-CELLS, INDUCED G(2) DELAY, mitosis, PROTEIN-KINASE, RADIOSENSITIZATION, STAUROSPORINE},
	isbn = {0022-3263},
	url = {<Go to ISI>://000077834800044},
	author = {Berlinck, R. G. S. and Britton, R. and Piers, E. and Lim, L. and Roberge, M. and da Rocha, R. M. and Andersen, R. J.}
}
@article {4389,
	title = {High-throughput assay for G(2) checkpoint inhibitors and identification of the structurally novel compound isogranulatimide},
	journal = {Cancer Research},
	volume = {58},
	number = {24},
	year = {1998},
	note = {ISI Document Delivery No.: 148HTTimes Cited: 83Cited Reference Count: 39},
	month = {Dec},
	pages = {5701-5706},
	type = {Article},
	abstract = {Treatment of cancer cells lacking p53 function with G(2) checkpoint inhibitors sensitizes them to the toxic effects of DNA damage and has been proposed as a strategy for cancer therapy. However, few inhibitors are known, and they have been found serendipitously, We report the development of a G(2) checkpoint inhibition assay that is suitable for high-throughput screening and its application to a screen of 1300 natural extracts. We present the isolation of a new G(2) checkpoint inhibitor, the structurally novel compound isogranulatimide. In combination with gamma-irradiation, isogranulatimide selectively kills MCF-7 cells lacking p53 function.},
	keywords = {ANTITUMOR DRUG, BHK CELLS, CAFFEINE, CELL-CYCLE CHECKPOINTS, DNA-DAMAGE, HELA-CELLS, mitosis, P34(CDC2) KINASE, P53 FUNCTION, PREMATURE, PROTEIN KINASE-C},
	isbn = {0008-5472},
	url = {<Go to ISI>://000077499800017},
	author = {Roberge, M. and Berlinck, R. G. S. and Xu, L. and Anderson, H. J. and Lim, L. Y. and Curman, D. and Stringer, C. M. and Friend, S. H. and Davies, P. and Vincent, I. and Haggarty, S. J. and Kelly, M. T. and Britton, R. and Piers, E. and Andersen, R. J.}
}
