@article {4236,
	title = {Stability and oligosaccharide binding of the N1 cellulose-binding domain of Cellulomonas fimi endoglucanase CenC},
	journal = {Biochemistry},
	volume = {37},
	number = {10},
	year = {1998},
	note = {ISI Document Delivery No.: ZC900Times Cited: 16Cited Reference Count: 75},
	month = {Mar},
	pages = {3529-3537},
	type = {Article},
	abstract = {Differential scanning calorimetry has been used to study the thermal stability and oligosaccharide-binding thermodynamics of the N-terminal cellulose-binding domain of Cellulomonas fimi beta-1,4-glucanase CenC (CBDN1) CBDN1 has a relatively low maximum stability (Delta G(max) = 33 kJ/mol = 216 J/residue at 1 degrees C and pH 6.1) compared to other small single-domain globular proteins. The unfolding is fully reversible between pH 5.5 and 9 and in accordance with the two-state equilibrium model between pH 5.5 and 11. When the single disulfide bond in CBDN1 is reduced, the protein remains unfolded at all conditions, as judged by NMR spectroscopy. This indicates that the intramolecular cross-link makes a major contribution to the stability of CBDN1. The measured heat capacity change of unfolding (Delta C-p = 7.5 kJ mol(-1) K-1) agrees well with that calculated from the predicted changes in the solvent accessible nonpolar and polar surface areas upon unfolding. Extrapolation of the specific enthalpy and entropy of unfolding to their respective convergence temperature indicates that per residue unfolding energies for CBDN1, an isolated domain, are in accordance with those found by Privalov (1) for many single-domain globular proteins. DSC thermograms of the unfolding of CBDN1 in the presence of various concentrations of cellopentaose were fit to a thermodynamic model describing the linkage between protein-ligand binding and protein unfolding. A global two-dimensional minimization routine is used to regress the binding enthalpy, binding constant, and unfolding thermodynamics for the CBDN1-cellopentaose system. Extrapolated binding constants are in quantitative agreement with those determined by isothermal titration calorimetry at 35 degrees C.},
	keywords = {ASPERGILLUS-NIGER, BRIDGES, differential scanning calorimetry, Disulfide, ESCHERICHIA-COLI, GLOBULAR-PROTEINS, INHIBITOR BINDING, LIGAND-BINDING, PROTEIN-FOLDING THERMODYNAMICS, THERMAL-DENATURATION, TITRATION CALORIMETRY},
	isbn = {0006-2960},
	url = {<Go to ISI>://000072630300034},
	author = {Creagh, A. L. and Koska, J. and Johnson, P. E. and Tomme, P. and Joshi, M. D. and McIntosh, L. P. and Kilburn, D. G. and Haynes, C. A.}
}
@article {3575,
	title = {COMPLEXES OF TRIVALENT METAL-IONS WITH POTENTIALLY HEPTADENTATE N4O3 SCHIFF-BASE AND AMINE PHENOL LIGANDS OF VARYING RIGIDITY},
	journal = {Inorganic Chemistry},
	volume = {34},
	number = {8},
	year = {1995},
	note = {ISI Document Delivery No.: QT603Times Cited: 67Cited Reference Count: 47},
	month = {Apr},
	pages = {2164-2178},
	type = {Article},
	abstract = {{The synthesis and characterization of several potentially heptadentate N4O3 Schiff bases and amine phenols, as well as a series of their mononuclear and dinuclear complexes with indium and the lanthanides are reported. Schiff bases containing imidazolidine rings were the products of the known condensation reaction of triethylenetetramine with 3 equiv of 5-substituted salicylaldehydes to form H(3)api (5-H-substituent), H(3)Clapi (5-Cl-substituent), or H(3)Brapi (5-Br-substituent); KBH4 reduction of these Schiff bases gave the appropriate isomeric N4O3 amine phenols H-3(1,2,4-btt) and H-3(1,1,4,-btt), as well as an acetone adduct, H-3(1,2,4-ahi). The Schiff bases reacted with 1 equiv of a lanthanide (Ln(3+)) nitrate to produce mononuclear nine-coordinated [Ln(H(3)Xapi)-(NO3)(3)] complex wherein the ligand adopts a tridentate capping coordination mode, whereas the amine phenols formed mononuclear seven-coordinate complexes with the lanthanides and indium; homodinuclear complexes [LnL]2 were also obtained with the Schiff bases. The X-ray structures of the Schiff bases H(3)api and H(3)Clapi, the mononuclear amine phenol complexes Yb(1,2,4-btt) . 0.5CH(3)OH and In(1,1,4-btt), and the homodinuclear Schiff base complex [La(Brapi)](2) . 2CHCl(3) have been determined. Crystals of H(3)api (C27H30N4O3) are monoclinic, space group P2(1)/a},
	keywords = {BRIDGES, CRYSTAL, ETHER, LANTHANIDE COMPLEXES, MOLECULAR-STRUCTURE, SALTS, TETRAHYDRATE, WATER, X-RAY},
	isbn = {0020-1669},
	url = {<Go to ISI>://A1995QT60300032},
	author = {Yang, L. W. and Liu, S. and Wong, E. and Rettig, S. J. and Orvig, Chris}
}
