@article {2121,
	title = {Making connections for life: an in vivo map of the yeast interactome},
	journal = {Hfsp Journal},
	volume = {2},
	number = {5},
	year = {2008},
	note = {ISI Document Delivery No.: 355VHTimes Cited: 1Cited Reference Count: 28Kast, Juergen},
	month = {Oct},
	pages = {244-250},
	type = {Article},
	abstract = {Proteins are the true workhorses of any cell. To carry out specific tasks, they frequently bind other molecules in their surroundings. Due to their structural complexity and flexibility, the most diverse array of interactions is seen with other proteins. The different geometries and affinities available for such interactions typically bestow specific functions on proteins. Having available a map of protein - protein interactions is therefore of enormous importance for any researcher interested in gaining insight into biological systems at the level of cells and organisms. In a recent report, a novel approach has been employed that relies on the spontaneous folding of complementary enzyme fragments fused to two different proteins to test whether these interact in their actual cellular context [Tarassov et al., Science 320, 1465-1470 (2008)]. Genome- wide application of this protein- fragment complementation assay has resulted in the first map of the in vivo interactome of Saccharomyces cerevisiae. The current data show striking similarities but also significant differences to those obtained using other large- scale approaches for the same task. This warrants a general discussion of the current state of affairs of protein - protein interaction studies and foreseeable future trends, highlighting their significance for a variety of applications and their potential to revolutionize our understanding of the architecture and dynamics of biological systems.},
	keywords = {AMINO-ACIDS, CELL-CULTURE, COMPLEXES, CROSS-LINKING, IDENTIFICATION, MASS-SPECTROMETRY, PROTEIN-PROTEIN INTERACTIONS, QUANTIFICATION, QUANTITATION, SACCHAROMYCES-CEREVISIAE},
	isbn = {1955-2068},
	url = {<Go to ISI>://000259736700002},
	author = {Kast, J.}
}
@article {998,
	title = {Identification and quantification of arsC genes in environmental samples by using real-time PCR},
	journal = {Journal of Microbiological Methods},
	volume = {58},
	number = {3},
	year = {2004},
	note = {ISI Document Delivery No.: 847SSTimes Cited: 24Cited Reference Count: 34},
	month = {Sep},
	pages = {335-349},
	type = {Article},
	abstract = {The arsC gene is responsible for the first step in arsenate biotransformation encoding the enzyme arsenate reductase. The quantitative real-time PCR method was developed to quantify the abundance of the arsC genes in environmental samples contaminated with arsenic. Two sets of primers that showed high specificity for the target arsC gene were designed based on consensus sequences from 13 bacterial species. The arsC gene was used as an external standard instead of total DNA in the calibration curve for real-time PCR, which was linear over six orders of magnitude and the detection limit was estimated to be about three copies of the gene. Soil samples from arsenic contaminated sites were screened for arsC genes by using PCR and showed the presence of this gene. The copy numbers of the gene ranging from 0.88 x 10(4) to 1.56 x 10(5) per ng total DNA were found in eight arsenic contaminated samples. Soil samples from a bioreactor containing pulp mill biomass and high concentration of arsenate showed a tenfold higher count of arsC gene copies than soil samples collected underground from an arsenic-rich gold mine. (C) 2004 Elsevier B.V. All rights reserved.},
	keywords = {arsC gene, arsenate reduction, ASSAY, BACTERIA, DNA, ESCHERICHIA-COLI, group-specific primers, QUANTITATION, real-time PCR, REDUCTION, resistance, SOIL, SYBR (R) Green 1},
	isbn = {0167-7012},
	url = {<Go to ISI>://000223415800006},
	author = {Sun, Y. M. and Polishchuk, E. A. and Radoja, U. and Cullen, W. R.}
}
@article {4199,
	title = {Active oxygen species formation in synaptosomes exposed to an aluminum chelator},
	journal = {Neurotoxicology and Teratology},
	volume = {20},
	number = {3},
	year = {1998},
	note = {ISI Document Delivery No.: ZU241Times Cited: 4Cited Reference Count: 37},
	month = {May-Jun},
	pages = {317-320},
	type = {Article},
	abstract = {This study evaluates the potential of two chelators, 1,2-dimethyl-3-hydroxypyridine-4-one (Hdpp) and 1-n-butyl-2-methyl-3-hydroxypyridin-4-one (Hnbp), to modulate cerebral rates of free radical production. The fluorometric assay for 2{\textquoteright},7{\textquoteright}-dichlorofluorescein, which is formed by oxidation of a nonfluorescent precursor (2{\textquoteright},7{\textquoteright}-dichlorofluorescin diacetate), was used to assay reactive oxygen species (ROS) production. The chelator Hdpp alone and the aluminum complexes of each chelator, Al(dpp)(3) and Al(nbp)(3), all inhibited basal rates of generation of ROS within a rat cerebral synaptosomal fraction. In the presence of an iron salt (1 mu M FeSO4), a major enhancement of syn aptosomal ROS formation was apparent. However, with the addition of an equimolar concentration of Hdpp, Al(dpp)(3), or Al(nbp)(3), this stimulation was completely abolished. The N-substituted-3-hydroxy-4-pyridinones have been proposed to be of clinical utility for the removal of iron or aluminum from tissues. The clinical potential of this class of chelator may be enhanced by their ability to inhibit iron-related oxidative events. (C) 1998 Elsevier Science Inc.},
	keywords = {3-HYDROXY-4-PYRIDINONES, aluminum, ALZHEIMERS-DISEASE, CHELATOR, COMPLEXES, DEFEROXAMINE, DESFERRIOXAMINE, Free Radicals, hydroxypyridinones, iron, LIPID-PEROXIDATION, metal chelation, NEUROFIBRILLARY TANGLES, QUANTITATION, RATS, reactive oxygen species},
	isbn = {0892-0362},
	url = {<Go to ISI>://000074176500011},
	author = {Bondy, S. C. and Tseng, H. and Orvig, Chris}
}
@article {3092,
	title = {SPECIATION OF ARSENIC COMPOUNDS BY HPLC WITH HYDRIDE GENERATION ATOMIC-ABSORPTION SPECTROMETRY AND INDUCTIVELY-COUPLED PLASMA-MASS SPECTROMETRY DETECTION},
	journal = {Talanta},
	volume = {41},
	number = {4},
	year = {1994},
	note = {ISI Document Delivery No.: NJ813Times Cited: 94Cited Reference Count: 45},
	month = {Apr},
	pages = {495-502},
	type = {Article},
	abstract = {An arsenic specific detection system utilizing on-line microwave digestion and hydride generation atomic absorption spectrometry (MD/HGAAS) is described for arsenic speciation by using high performance liquid chromatography (HPLC). Both ion exchange chromatography and ion pair chromatography have been studied for the separation of arsenite, arsenate, monomethylarsonic acid (MMAA), dimethylarsinic acid (DMAA), and arsenobetaine (AB). When the commonly used mobile phases, phosphate and carbonate buffers at pH 7.5, are used on an anion exchange column, arsenite and AB co-elute. However, selective determination of these two arsenic compounds can be achieved by using the new detection system. Partial separation between arsenite and AB can be achieved by increasing the mobile phase pH to 10.3 and by using a polymer based anion exchange column. The detection limit obtained by using anion exchange chromatography with MD/HGAAS detection is approximately 10 ng/ml (or 200 pg for a 20-mul sample injection) for arsenite, DMAA and AB, 15 ng/ml (or 300 pg) for MMAA, and 20 ng/ml (or 400 pg) for arsenate. Complete separation of the five arsenic compounds is achieved on a reversed phase C18 column by using sodium heptanesulfonate as ion pair reagent. Comparable resolution between chromatographic peaks is obtained by using MD/HGAAS detection and inductively coupled plasma mass spectrometry (ICPMS) detection.},
	keywords = {ELEMENT-SPECIFIC DETECTOR, IDENTIFICATION, ION CHROMATOGRAPHY, MUSCLE, ORGANOARSENIC COMPOUNDS, PERFORMANCE LIQUID-CHROMATOGRAPHY, QUANTITATION, REFERENCE MATERIAL, SEPARATION, TRACE-ELEMENTS, WATER},
	isbn = {0039-9140},
	url = {<Go to ISI>://A1994NJ81300003},
	author = {Le, X. C. and Cullen, W. R. and Reimer, K. J.}
}
@article {7295,
	title = {DECOMPOSITION OF ORGANOARSENIC COMPOUNDS BY USING A MICROWAVE-OVEN AND SUBSEQUENT DETERMINATION BY FLOW-INJECTION HYDRIDE GENERATION ATOMIC-ABSORPTION SPECTROMETRY},
	journal = {Applied Organometallic Chemistry},
	volume = {6},
	number = {2},
	year = {1992},
	note = {ISI Document Delivery No.: HQ168Times Cited: 61Cited Reference Count: 33INTERNATIONAL CONF ON ENVIRONMENTAL AND BIOLOGICAL ASPECTS OF MAIN-GROUP ORGANOMETALS ( ICEBAMO )SEP 15-19, 1991PADUA, ITALYUNIV PADUA, ITALIAN CHEM SOC},
	month = {Apr},
	pages = {161-171},
	type = {Proceedings Paper},
	abstract = {Environmentally important organoarsenicals such as arsenobetaine, arsenocholine and tetramethylarsonium ion do not form volatile hydrides under the commonly used analytical conditions on treatment with borohydride and it has been difficult to determine their concentrations without further derivatization. This paper describes a rapid method which completely decomposes and oxidizes these arsenicals to arsenate by using potassium persulphate and sodium hydroxide with the aid of microwave energy. The quantitative decomposition of these species permits their determination at low nanogram levels, by hydride generation atomic absorption spectrometry (HG AA). A new hydride generator which has high efficiency and minimum dead volume and therefore is suitable for flow injection analysis (FIA) is also described. A system combining flow injection analysis, on-line microwave oven digestion, and hydride generation followed by atomic absorption measurement, is developed. This system is capable of performing analysis at a sample throughput of 100-120 per hour. Calibration curves were linear from 10 to 200 ng cm-3 of arsenic and the detection limit was 5 ng cm-3 for a 100-mu-l injection or 0.5 ng of arsenic. All ten organoarsenic compounds studied gave arsenate as the decomposition product, which was confirmed by using molybdenum blue photometric measurement.},
	keywords = {ABSORPTION SPECTROMETRY, arsenic, arsenobetaine, atomic, DECOMPOSITION, DETERMINATION, DISSOLUTION, ENVIRONMENT, FLOW INJECTION ANALYSIS, HYDRIDE GENERATION, IDENTIFICATION, LIQUID-CHROMATOGRAPHY, MICROWAVE OVEN DIGESTION, MUSCLE REFERENCE MATERIAL, QUANTITATION, TRACE-ELEMENTS, WATER},
	isbn = {0268-2605},
	url = {<Go to ISI>://A1992HQ16800008},
	author = {Le, X. C. and Cullen, W. R. and Reimer, K. J.}
}
