@article {2137,
	title = {Synthesis and Enzymatic Incorporation of Modified Deoxyadenosine Triphosphates},
	journal = {European Journal of Organic Chemistry},
	number = {29},
	year = {2008},
	note = {ISI Document Delivery No.: 365YXTimes Cited: 7Cited Reference Count: 35Lam, Curtis Hipolito, Christopher Perrin, David M.},
	month = {Oct},
	pages = {4915-4923},
	type = {Article},
	abstract = {Several deoxyadenosine triphosphates containing modifications at the 8-position have been synthesized. Suitably protected 8-bromodeoxyadenosines were coupled with five imidazole-containing moieties by nucleophilic aromatic substitution or Sonagashira coupling to give modified nucleosides that were then triphosphorylated. Incorporation assays were performed for these modified residues with many commercially available DNA polymerases, and it was found that two of the modified dATPs could be effectively taken up as substrates by Sequenase V2.0. These two residues are candidates for substrates in combinatorial selections in the search for improved catalysis from DNAzymes. ((C) Wiley-VCH Verlag GmbH \& Co. KGaA, 69451 Weinheim, Germany, 2008)},
	keywords = {AMINO-ACID, bioorganic chemistry, CATALYTIC REPERTOIRE, CLEAVING DNA ENZYME, DERIVATIVES, DNA, ENZYMES, FUNCTIONALITIES, HETEROCYCLES, IN-VITRO SELECTION, NUCLEIC-ACIDS, nucleotides, OLIGONUCLEOTIDES, PCR, POLYMERASES, SELEX},
	isbn = {1434-193X},
	url = {<Go to ISI>://000260447200009},
	author = {Lam, C. and Hipolito, C. and Perrin,David M.}
}
@article {429,
	title = {Incorporation of 8-histaminyldeoxyadenosine [8-(2-(4-imidazolyl)ethylamino)-2 {\textquoteright}-deoxyriboadenosine] into oligodeoxyribonucleotides by solid phase phosphoramidite coupling},
	journal = {Nucleosides Nucleotides \& Nucleic Acids},
	volume = {21},
	number = {10},
	year = {2002},
	note = {ISI Document Delivery No.: 622CATimes Cited: 5Cited Reference Count: 37},
	pages = {651-664},
	type = {Article},
	abstract = {The 3{\textquoteright} phosphoramidite of 8-histaminyl deoxyadenosine has been prepared and successfully incorporated into a short oligodeoxyribonucleotide. The synthetic methodology leading to this preparation is given and the implications for developing new DNAzymes as well as probing unusual nucleic acid structures are discussed.},
	keywords = {BEARING IMIDAZOLE, CATALYTIC REPERTOIRE, CLEAVAGE, DERIVATIVES, DNA, IMIDAZOLYL FUNCTIONALITIES, NUCLEIC-ACIDS, OLIGONUCLEOTIDES, RIBONUCLEASES, RNA},
	isbn = {1525-7770},
	url = {<Go to ISI>://000179627900003},
	author = {Lermer, L. and Hobbs, J. and Perrin,David M.}
}
@article {5031,
	title = {Artificial nucleases},
	journal = {Chembiochem},
	volume = {2},
	number = {10},
	year = {2001},
	note = {ISI Document Delivery No.: 479NGTimes Cited: 54Cited Reference Count: 25},
	month = {Oct},
	pages = {735-740},
	type = {Article},
	abstract = {The oxidation of DNA and RNA provides a facile approach for investigating the interaction of nucleic acids wit proteins and oligonucleotides In this article, we have outlined our understanding of the mechanism of DNA scission by 1,10-phenanthroline-copper(I) in the presence of hydrogen peroxide. We also discuss results obtained by using 1,10-phenanthroline - oligonucleotide conjugates in probing the size of the transcriptionally active open complex. Finally, we outline an effective method for converting DNA-binding proteins into site-specific modification agents by using 1,10-phenanthroline-copper(I).},
	keywords = {1, 10-PHENANTHROLINE-COPPER ION, chemical, COLI RNA-POLYMERASE, COMPLEX, copper, DNA, DNA cleavage, DNA recognition, ESCHERICHIA-COLI, NUCLEASE, nucleic acids, OLIGONUCLEOTIDES, PROTEIN, SITE-SPECIFIC NUCLEASE, TRANSCRIPTION INITIATION},
	isbn = {1439-4227},
	url = {<Go to ISI>://000171410000003},
	author = {Chen, C. H. B. and Milne, L. and Landgraf, R. and Perrin,David M. and Sigman, D. S.}
}
@article {4920,
	title = {Electrospray ionization of alkali and alkaline earth metal species. Electrochemical oxidation and pH effects},
	journal = {Journal of Mass Spectrometry},
	volume = {35},
	number = {8},
	year = {2000},
	note = {ISI Document Delivery No.: 347MUTimes Cited: 7Cited Reference Count: 49},
	month = {Aug},
	pages = {981-989},
	type = {Article},
	abstract = {The utility of electrospray ionization mass spectrometry (ESI-MS) for characterizing dissolved metal species has generated considerable interest in the use of this technique for metal speciation, However, the development of accurate speciation methods based on ESI-R;IS requires a detailed understanding of the mechanisms by which dissolved metal species are ionized during electrospray. We report how the analysis of alkali and alkaline earth metal species pro,ides new information about some of the processes that affect electrospray ion yield. Selected metal ions and organic ligands were combined in 50:50 water-acetonitrile buffered with acetic acid or ammonium acetate and analyzed by flow injection ESI-MS using mild electrospray conditions. Species formed by alkali metal ions with thiol and oxygen-donating ligands were detected in acidic and neutral pH solutions. Electrochemical oxidation of N,N-diethyldithiocarbamate and glutathione during electrospray was indicated by detection of the corresponding disulfides as protonated or alkali metal species, The extent of ligand oxidation depended on solution pH and the dissociation constant of the thiol group, Tandem mass spectrometric experiments suggested that radical cations such as [NaL](+.) (where L = N,N -diethyldithiocarbamate) can be generated by in-source fragmentation of disulfide species. Greater complexation of alkali metals at neutral pH tvas indicated by a corresponding decrease in the relative abundance of the free metal ion, The number of alkali metal ions bound by glutathione and phthalic acid also increased with increasing pH, in accordance with thermodynamic equilibrium theory. Alkaline earth metal species were detected only in acidic solutions, the absence of 8-hydroxyquinoline complexes being attributed to their relative instability and subsequent dissociation during electrospray, Hence, accurate speciation by ESI-MS depends on experimental conditions and the intrinsic properties of each analyte, Copyright (C) 2000 John Wiley \&; Sons, Ltd.},
	keywords = {cell, COMPLEXES, electrochemical oxidation, ELECTROPHORESIS, electrospray ionization, ION-TRAP, MASS-SPECTROMETRY, metal speciation, OLIGONUCLEOTIDES, PEPTIDES, pH effects, PROTEINS, STOICHIOMETRY, thiol ligands, WATER},
	isbn = {1076-5174},
	url = {<Go to ISI>://000088934400006},
	author = {Ross, A. R. S. and Ikonomou, M. G. and Orians, K. J.}
}
