@article {940,
	title = {Covalent Schiff base catalysis and turnover by a DNAzyme: A M2+ independent AP-endonuclease mimic},
	journal = {Journal of the American Chemical Society},
	volume = {126},
	number = {13},
	year = {2004},
	note = {ISI Document Delivery No.: 809LBTimes Cited: 44Cited Reference Count: 117},
	month = {Apr},
	pages = {4145-4156},
	type = {Review},
	abstract = {A DNAzyme, synthetically modified with both primary amines and imidazoles, is found to act as a M2+ -independent AP lyase-endonuclease. In the course of the cleavage reaction, this DNAzyme forms a covalent Schiff base intermediate with an abasic site on a complementary oligodeoxyribonucleotide. This intermediate, which is inferred from NaCNBH3 trapping as well as cyanide inhibition, does not evidently accumulate because the second step, dehydrophosphorylative elimination, is fast compared to Schiff base formation. The 5{\textquoteright}-product that remains linked to the catalyst hydrolyzes slowly to regenerate free catalyst. The use of duly modified DNAzymes to perform Schiff base catalysis demonstrates the value of modified nucleotides for enhancing the catalytic repertoire of nucleic acids. This work suggests that DNAzymes will be capable of catalyzing aldol condensation reactions.},
	keywords = {ALDEHYDIC ABASIC, APURINIC SITES, BETA-ELIMINATION, DIVALENT METAL-ION, FUNCTIONALITIES, IMIDAZOLYL, IN-VITRO SELECTION, NUCLEIC-ACIDS, RNA CLEAVAGE, SITES, TETRAHYMENA RIBOZYME, THERMOSTABLE DNA-POLYMERASES},
	isbn = {0002-7863},
	url = {<Go to ISI>://000220637100042},
	author = {May, J. P. and Ting, R. and Lermer, L. and Thomas, J. M. and Roupioz, Y. and Perrin,David M.}
}
@article {1016,
	title = {Selection and characterization of DNAzymes with synthetically appended functionalities: A case of a synthetic RNaseA mimic},
	journal = {Pure and Applied Chemistry},
	volume = {76},
	number = {7-8},
	year = {2004},
	note = {ISI Document Delivery No.: 855WQTimes Cited: 1Cited Reference Count: 7039th IUPAC Congress/86th Conference of the Canadian-Society-for-ChemistryAUG 10-15, 2003Ottawa, CANADACanadian Soc Chem, Natl Res Council Canada, Int Union Pure \& Appl Chem},
	month = {Jul-Aug},
	pages = {1571-1577},
	type = {Proceedings Paper},
	abstract = {We have been interested in merging synthetic nucleotide chemistry with combinatorial selection of DNAzymes to deliver a more complete (and complex) chemical complement to the catalytic repertoire of nucleic acids. Thus we ask, what do modified dNTPs really bring to nucleic acids in terms of an increased repertoire? In asking this question, we have looked first at conditions, and more recently for reaction classes where nucleic acids are found to be catalytically inefficient, deficient, or at least to date, seemingly incapable of certain functions. A case of this is M2+-independent ribophosphodiester hydrolysis at physiological pH and low ionic strength where nucleic acids exhibit especially low rate constants for self-cleavage and seem to be incapable of turnover.},
	keywords = {CATALYTIC, CLEAVING DNA ENZYME, DIVALENT METAL-ION, IMIDAZOLYL FUNCTIONALITIES, IN-VITRO SELECTION, MONOVALENT CATIONS, NUCLEIC-ACIDS, NUCLEOSIDE 5{\textquoteright}-TRIPHOSPHATES, REPERTOIRE, SEQUENCE-SPECIFIC CLEAVAGE, SITE-SPECIFIC CLEAVAGE},
	isbn = {0033-4545},
	url = {<Go to ISI>://000224006000028},
	author = {Ting, R. and Lermer, L. and Thomas, J. and Roupioz, Y. and Perrin,David M.}
}
