Research & Teaching Faculty

Separation of Recombinant Therapeutic Proteins Using Capillary Gel Electrophoresis and Capillary Isoelectric Focusing

TitleSeparation of Recombinant Therapeutic Proteins Using Capillary Gel Electrophoresis and Capillary Isoelectric Focusing
Publication TypeBook Chapter
Year of Publication2016
AuthorsDe Jong, CA, Risley, J, Lee, AK, Zhao, SS, Chen, DDY
Book TitleCapillary Electrophoresis of Proteins and Peptides
Chapter11
Pagination137-149
PublisherSpringer Nature
CityNew York
ISBN Number978-1-4939-4012-7
KeywordsCapillary gel electrophoresis; Capillary isoelectric focusing; Protein characterization; Recombinant therapeutic proteins
Abstract

Detailed step-by-step methods for protein separation techniques based on capillary electrophoresis (CE) are described in this chapter. Focus is placed on two techniques, capillary gel electrophoresis (CGE) and capillary isoelectric focusing (cIEF). CGE is essentially gel electrophoresis, performed in a capillary, where a hydrogel is used as a sieving matrix to separate proteins or peptides based on size. cIEF separates proteins or peptides based on their isoelectric point (pI), the pH at which the protein or peptide bears no charges. Detailed protocols and steps (including capillary preparation, sample preparation, CE separation conditions, and detection) for both CGE and cIEF presented so that readers can follow the described methods in their own labs.