|Title||Ceratamines, structurally simple microtubule-stabilizing antimitotic agents with unusual cellular effects|
|Publication Type||Journal Article|
|Year of Publication||2005|
|Authors||Karjala, G, Chan, Q, Manzo, E, Andersen, RJ, Roberge, N|
|Type of Article||Article|
|Keywords||BIND, CELLS, RESISTANT, TAXOID SITE, TUBULIN|
Ceratamine A and ceratamine B are heterocyclic alkaloids recently identified in a screen for compounds that arrest cells in mitosis. Treatment of breast carcinoma MCF-7 cells causes a concentration-dependent block of cell cycle progression exclusively at mitosis. In vitro studies with purified tubulin indicate that the ceratamines directly stimulate microtubule polymerization in the absence of microtubule-associated proteins. Cells treated with ceratamines show a dense perinuclear microtubule network in interphase and multiple pillar-like tubulin structures in mitotic cells. The ceratamines do not compete with paclitaxel for binding to microtubules in vitro. Unlike other microtubule-stabilizing agents, the ceratamines have simple structures with no chiral centers, making them attractive drug leads.
|URL||<Go to ISI>://000228424800012|