| Title | Crystallization and preliminary X-ray analysis of a xyloglucan endotransglycosylase from Populus tremula x tremuloides |
| Publication Type | Journal Article |
| Year of Publication | 2003 |
| Authors | Johansson, P, Denman, S, Brumer, H, Kallas, AM, Henriksson, H, Bergfors, T, Teeri, TT, Jones, TA |
| Journal | ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY |
| Volume | 59 |
| Pagination | 535-537 |
| Date Published | MAR |
| Type of Article | Article |
| ISSN | 0907-4449 |
| Abstract | {Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls. Recombinant XET from poplar has been purified from a Pichia pastoris expression system and crystallized. Two different crystal forms were obtained by vapour diffusion from potassium sodium tartrate and from an imidazole buffer using sodium acetate as a precipitant. Data were collected from these crystal forms to 3.5 and 2.1 Angstrom resolution, respectively. The first crystal form was found to belong to space group P3(1)21 or P3(2)21 (unit-cell parameters a = 98.6 |
| DOI | 10.1107/S090744490202348X |
