Title | DISTINGUISHING XYLOGLUCANASE ACTIVITY IN ENDO-beta(1 -> 4)GLUCANASES |
Publication Type | Book Chapter |
Year of Publication | 2012 |
Authors | Eklof, JM, Ruda, MC, Brumer, H |
Editor | Gilbert, HJ |
Book Title | CELLULASES |
Series Title | Methods in Enzymology |
Volume | 510 |
Pagination | 97-120 |
ISBN Number | 978-0-12-415931-0 |
ISBN | 0076-6879 |
Abstract | The ability of beta-glucanases to cleave xyloglucans, a family of highly decorated beta-glucans ubiquitous in plant biomass, has traditionally been overlooked in functional biochemical studies. An emerging body of data indicates, however, that a spectrum of xyloglucan specificity resides in diverse glycoside hydrolases from a range of carbohydrate-active enzyme families including classic ``cellulase{''} families. This chapter outlines a series of enzyme kinetic and product analysis methods to establish degrees of xyloglucan specificity and modes of action of glycosidases emerging from enzyme discovery projects. |
DOI | 10.1016/B978-0-12-415931-0.00006-9 |