| Title | Identification of the catalytic nucleophile of endoglucanase I from Fusarium oxysporum by mass spectrometry |
| Publication Type | Journal Article |
| Year of Publication | 1997 |
| Authors | MacKenzie, LF, Davies, GJ, Schulein, M, Withers, SG |
| Journal | BIOCHEMISTRY |
| Volume | 36 |
| Pagination | 5893-5901 |
| Date Published | MAY 13 |
| ISSN | 0006-2960 |
| Abstract | {The endoglucanase EG I from Fusarium oxysporum catalyzes the hydrolysis of cellulose via a double-displacement mechanism involving the formation and hydrolysis of a glycosyl-enzyme intermediate. Treatment of EG I with 2',4'-dinitrophenyl-2-deoxy-2-fluoro-beta-D-cellobioside results in the time-dependent inactivation of the enzyme (k(i) = 1.36 min(-1) |
| DOI | 10.1021/bi962962h |
