| Title | The inhibition of glutamate racemase by D-N-hydroxyglutamate |
| Publication Type | Journal Article |
| Year of Publication | 1997 |
| Authors | Glavas, S, Tanner, ME |
| Journal | Bioorganic & Medicinal Chemistry Letters |
| Volume | 7 |
| Pagination | 2265-2270 |
| Date Published | Sep |
| Type of Article | Article |
| ISBN Number | 0960-894X |
| Keywords | CLONING, DIAMINOPIMELIC ACID, EPIMERASE, ESCHERICHIA-COLI, GENE, L-ALANINE, LACTOBACILLUS, MURI, PEDIOCOCCUS-PENTOSACEUS, resistance |
| Abstract | We report that D-N-hydroxyglutamate is ii competitive inhibitor of glutamate racemase (K-I=56 mu M). The compound acts as an alternate substrate and is converted into a-ketoglutarate and ammonia (K-M=57 mu M, k(cat)/K-M=3.2x10(3) M(-1)sec(-1)), An imine intermediate is likely the species causing the inhibition. L-N-hydroxyglutamate is a weak inhibitor and is slowly converted to alpha-ketoglutarate (k(cat)/K-M=30 M(-1)sec(-1)). (C) 1997 Elsevier Science Ltd. |
| URL | <Go to ISI>://A1997XX50700025 |
