| Title | KORRIGAN1 and its Aspen Homolog PttCel9A1 Decrease Cellulose Crystallinity in Arabidopsis Stems |
| Publication Type | Journal Article |
| Year of Publication | 2009 |
| Authors | Takahashi, J, Rudsander, UJ, Hedenstrom, M, Banasiak, A, Harholt, J, Amelot, N, Immerzeel, P, Ryden, P, Endo, S, Ibatullin, FM, Brumer, H, del Campillo, E, Master, ER, Scheller, HVibe, Sundberg, B, Teeri, TT, Mellerowicz, EJ |
| Journal | PLANT AND CELL PHYSIOLOGY |
| Volume | 50 |
| Pagination | 1099-1115 |
| Date Published | JUN |
| Type of Article | Article |
| ISSN | 0032-0781 |
| Abstract | KORRIGAN1 (KOR1) is a membrane-bound cellulase implicated in cellulose biosynthesis. PttCel9A1 from hybrid aspen (Populus tremula L. tremuloides Michx.) has high sequence similarity to KOR1 and we demonstrate here that it complements kor1-1 mutants, indicating that it is a KOR1 ortholog. We investigated the function of PttCel9A1/KOR1 in Arabidopsis secondary growth using transgenic lines expressing 35S::PttCel9A1 and the KOR1 mutant line irx2-2. The presence of elevated levels of PttCel9A1/KOR1 in secondary walls of 35S::PttCel9A1 lines was confirmed by in muro visualization of cellulase activity. Compared with the wild type, 35S::PttCel9A1 lines had higher trifluoroacetic acid (TFA)-hydrolyzable glucan contents, similar Updegraff cellulose contents and lower cellulose crystallinity indices, as determined by (13)C solid-state nuclear magnetic resonance (NMR) spectroscopy. irx2-2 mutants had wild-type TFA-hydrolyzable glucan contents, but reduced Updegraff cellulose contents and higher than wild-type cellulose crystallinity indices. The data support the hypothesis that PttCel9A1/KOR1 activity is present in cell walls, where it facilitates cellulose biosynthesis in a way that increases the amount of non-crystalline cellulose. |
| DOI | 10.1093/pcp/pcp062 |
