CHEM 434/534 (3 Units)
Principles of chemical separation and Mass Spectrometry
Unified separation science, liquid and gas chromatography, capillary electrophoresis, Optical and mass spectroscopic detection, separation methods coupled with mass spectrometry and tandem mass spectrometry, biochemical and multi-omics applications.
Rational for a chemical separation course at the 400 and 500 levels
The advancement in chemical separation techniques is a crucial contributing factor in the development of pure and applied sciences. However, except for a very rudimentary introduction for chemical separation techniques in Chem 311 (Instrumental Chemical Analysis), this very important area analytical chemistry has never been taught to the undergraduate students at UBC. A graduate level course, Chem 534, has already in existence for over 30 years, so the creation of a combined graduate and undergraduate course is aimed to teach the widely used separation techniques to both graduate and undergraduate students, and make them better prepared for their potential fields of employment or continue in their graduate studies and research.
Course Schedule
| Week | Topics |
|---|---|
| 1 | Unified separation science |
| 2 | Liquid chromatography |
| 3 | Gas chromatography |
| 4 | Capillary electrophoresis |
| 5 | Microfluidics |
| 6 | Detection techniques |
| 7 | Mass spectrometry in separation techniques |
| 8 | Applications of LC |
| 9 | DNA Sequencing Technologies |
| 10 | Protein Sequencing Technologies |
| 11 | LC-MS and CE-MS applications in multi-omics |
| 12 | Future trends in separation science |
| 13 | Separation science and biotechnology and pharma Industry |
Learning Outcomes
The students will learn the principles driving all separation techniques, the principles of operation of the separation instruments, mass spectrometry instrument and applications, the applications of chemical separation and mass spectrometry in academic research and chemical and pharmaceutical industry.
Assessment Criteria and Grading
20% homework, 20% midterm, 20% term paper, and 40% Final
Assigned Readings
“Principles of Instrumental Analysis” by Douglas A. Skoog, F. James Holler, Stanley R. Crouch, “Unified Separation Science” by J. C. Giddings, and a Collection of research papers