|Title||Synthesis and characterisation of novel chromogenic substrates for human pancreatic alpha-amylase|
|Publication Type||Journal Article|
|Year of Publication||2004|
|Authors||Damager, I, Numao, S, Chen, HM, BRAYER, GD, Withers, SG|
|Date Published||JUL 12|
Derivatives of maltose and maltotriose were chemically synthesised as substrates for human pancreatic alpha-amylases and subjected to kinetic analysis. Rates measured were shown to reflect both hydrolysis and transglycosylation reactions. 4-O-Methylated derivatives of these substrates underwent only hydrolysis, thereby simplifying kinetic analyses. These modified substrates may be used for the detection and kinetic analysis of a-amylases, and are useful in rapidly screening for novel a-amylase inhibitors and for subsequent kinetic characterisation. (C) 2004 Published by Elsevier Ltd.