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  3. ARSENIC BIOMETHYLATION BY THE MICROORGANISM APIOTRICHUM HUMICOLA IN THE PRESENCE OF L-METHIONINE-METHYL-D(3)

ARSENIC BIOMETHYLATION BY THE MICROORGANISM APIOTRICHUM HUMICOLA IN THE PRESENCE OF L-METHIONINE-METHYL-D(3)

TitleARSENIC BIOMETHYLATION BY THE MICROORGANISM APIOTRICHUM HUMICOLA IN THE PRESENCE OF L-METHIONINE-METHYL-D(3)
Publication TypeJournal Article
Year of Publication1995
AuthorsCullen, WR, Li, H, Pergantis, SA, Eigendorf, GK, Mosi, AA
JournalApplied Organometallic Chemistry
Volume9
Pagination507-515
Date PublishedNov
Type of ArticleProceedings Paper
ISBN Number0268-2605
KeywordsARSENATE, ARSENITE, CHROMATOGRAPHY MASS SPECTROMETRY, DIMETHYLARSINIC ACID, EXTRACELLULAR, GAS, HYDRIDE GENERATION, HYDRIDE GENERATION GAS, L-METHIONINE-METHYL-D(3), methylarsonic acid, METHYLATION, MICROORGANISM, REDUCTION, S-ADENOSYLMETHIONINE, THIOLS, TRIMETHYLARSINE OXIDE
Abstract

The microorganism Apiotrichum humicola (previously known as Candida humicola) grown in the presence of either arsenate, arsenite, methyl-arsonic acid or dimethylarsinic acid, produces arsenic-containing metabolites in the growth medium. When L-methionine-methyl-d(3) is added to the cultures, the CD3 label is incorporated intact into the metabolites to a considerable extent to form deuterated dimethylarsenic and trimethylarsenic species, indicating that S-adenosylmethionine, or some related sulphonium compound, is involved in the biological methylation. Conclusive evidence of CD3 incorporation in the arsenicals found in the growth medium was provided by using a specially developed hydride generation-gas chromatography-mass spectrometry system (HG-GC-MS).
URL<Go to ISI>://A1995TG18900002