Title | Ceratamines, structurally simple microtubule-stabilizing antimitotic agents with unusual cellular effects |
Publication Type | Journal Article |
Year of Publication | 2005 |
Authors | Karjala, G, Chan, Q, Manzo, E, Andersen, RJ, Roberge, N |
Journal | Cancer Research |
Volume | 65 |
Pagination | 3040-3043 |
Date Published | Apr |
Type of Article | Article |
ISBN Number | 0008-5472 |
Keywords | BIND, CELLS, RESISTANT, TAXOID SITE, TUBULIN |
Abstract | Ceratamine A and ceratamine B are heterocyclic alkaloids recently identified in a screen for compounds that arrest cells in mitosis. Treatment of breast carcinoma MCF-7 cells causes a concentration-dependent block of cell cycle progression exclusively at mitosis. In vitro studies with purified tubulin indicate that the ceratamines directly stimulate microtubule polymerization in the absence of microtubule-associated proteins. Cells treated with ceratamines show a dense perinuclear microtubule network in interphase and multiple pillar-like tubulin structures in mitotic cells. The ceratamines do not compete with paclitaxel for binding to microtubules in vitro. Unlike other microtubule-stabilizing agents, the ceratamines have simple structures with no chiral centers, making them attractive drug leads. |
URL | <Go to ISI>://000228424800012 |