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Collision cross sections of gas phase DNA ions

TitleCollision cross sections of gas phase DNA ions
Publication TypeJournal Article
Year of Publication2002
AuthorsMoradian, A, Scalf, M, Westphall, MS, Smith, LM, Douglas, DJ
JournalInternational Journal of Mass Spectrometry
Volume219
Pagination161-170
Date PublishedAug
Type of ArticleArticle
ISBN Number1387-3806
Keywordscollision cross sections, CONFORMATION, CONFORMERS, DNA ions, ELECTROSPRAY MASS-SPECTROMETRY, gas phase, MYOGLOBIN, PROTEIN IONS, SEQUENCE, VACUUM
Abstract

Collision cross sections of negative ions of a 28-, 40- and 55-mer of single stranded DNA have been measured by an energy loss method, and compared to collision cross sections of proteins of nearly the same molecular weight-ubiquitin, cytochrome c and apomyoglobin, respectively. The oligonucleotides produce negative charge states in electrospray ionization (ESI) similar to the positive charge states produced by the proteins denatured in solution. Cross sections for deoxynucleotide ions increase with charge state in a manner similar to those of protein ions. However, for a given molecular weight and charge state, the cross sections of the oligodeoxynucleotide ions are about 22% lower than those of the proteins. (C) 2002 Elsevier Science B.V. All rights reserved.

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