|Title||Pulling Genetic RNA out of Tobacco Mosaic Virus Using Single-Molecule Force Spectroscopy|
|Publication Type||Journal Article|
|Year of Publication||2010|
|Authors||Liu, N, Peng, B, Lin, Y, Su, Z, Niu, Z, Wang, Q, Zhang, W, Li, H, Shen, J|
|Journal||JOURNAL OF THE AMERICAN CHEMICAL SOCIETY|
|Date Published||AUG 18|
RNA-coat protein interactions in intact tobacco mosaic virus have been investigated for the first time directly on the single-molecule level by pulling the genetic RNA step by step out of the helical groove formed by its protein coat. The effects of pulling speed and pH on RNA-protein interactions are presented. In addition, the rebinding behavior of the detached RNA with the protein coat is discussed. Our results demonstrate the possibility of studying nucleic acid-protein interactions in more complicated systems using AFM-based single-molecule force spectroscopy.