|Title||Rice BGlu1 glycosynthase and wild type transglycosylation activities distinguished by cyclophellitol inhibition|
|Publication Type||Journal Article|
|Year of Publication||2012|
|Authors||Pengthaisong, S, Chen, C-F, Withers, SG, Kuaprasert, B, Cairns, JRKetudat|
|Date Published||MAY 1|
The rice BGlu1 beta-D-glucosidase nucleophile mutant E386G is a glycosynthase that catalyzes the synthesis of cellooligosaccharides from alpha-D-glucopyranosyl fluoride (GlcF) donor and p-nitrophenyl (pNP) cellobioside (Glc2-pNP) or cello-oligosaccharide acceptors. When activity with other donors and acceptors was tested, the initial enzyme preparation cleaved pNP-beta-D-glucopyranoside (Glc-pNP) and pNP-beta-D-fucopyranoside (Fuc-pNP) to pNP and glucose and fucose, suggesting contamination with wild type BGlu1 beta-glucosidase. The products from reaction of GlcF and Fuc-pNP included Fuc-beta-(1 -> 3)-Fuc-pNP, Glc-beta-(1 -> 3)Fuc-pNP, and Fuc-beta-(1 -> 4)-Glc-beta-(1 -> 3)-Fuc-pNP, suggesting the presence of both wild type BGlu1 and its glycosynthase. Inhibition of the BGlu1 beta-glucosidase activity within this preparation by cyclophellitol confirmed that the E386G glycosynthase preparation was contaminated with wild type BGlu1. Rice BGlu1 E386G-2, generated from a new construct designed to minimize back-mutation, showed glycosynthase activity without wild type hydrolytic or transglycosylation activity. E386G-2 catalyzed transfer of glycosyl residues from GlcF, alpha-L-arabinosyl fluoride, alpha-D-fucosyl fluoride, alpha-D-galactosyl fluoride, alpha-D-mannosyl fluoride, and alpha-D-xylosyl fluoride donors to Glc2-pNP acceptor. The synthetic products from the reactions of alpha-fucosyl fluoride and alpha-mannosyl fluoride donors were confirmed to result from addition of a beta-(1 -> 4)-linked glycosyl residue. Moreover, the E386G glycosynthase transferred glucose from GlcF donor to glucose, cellobiose, Glc-pNP, Fuc-pNP, pNP-beta-D-galactopyranoside, and pNP-beta-D-xylopyranoside acceptors, but little to pNP-beta-D-mannopyranoside. Production of longer oligosaccharides occurred most readily on acceptors with an equatorial 4-OH. Elimination of wild type contamination thereby allowed a clear assessment of BGlu1 E386G glycosynthase catalytic abilities. (C) 2012 Elsevier Ltd. All rights reserved.