Title | Application of multisegment injection on quantification of creatinine and standard addition analysis of urinary 5-hydroxyindoleacetic acid simultaneously with creatinine normalization |
Publication Type | Journal Article |
Year of Publication | 2020 |
Authors | Huang, Z-A, Scotland, KB, Li, Y, Guo, J-P, McGeer, PL, Lange, D, Chen, DDY |
Journal | ELECTROPHORESIS |
Volume | 41 |
Pagination | 183-193 |
Keywords | capillary electrophoresis, creatinine normalization, MASS SPECTROMETRY, multisegment injection, urinary 5-hydroxyindoleacetic acid |
Abstract | Abstract In this paper, the development of a simple dilute-and-shoot method for quantifying urinary creatinine by CE–ESI–MS was described. The creatinine analysis time was about 7 min/sample by conventional single injection (SI) method and can be significantly reduced to less than 2 min/sample with multi-segment injection (MSI). In addition, the standard addition analysis of 5-hydroxyindole-3-acetic acid (5-HIAA) and creatinine normalization was performed within one run by the MSI technique, and the total analysis time was 14-min faster compared to the SI method for analyzing the same set of samples. The uses of isotopic and non-isotopic internal standards (ISs) were compared. Creatinine-(methyl-13C) and 5-hydroxyindole-4,6,7-D3-3-acetic-D2 acid (5-HIAA-D5) used as isotopic ISs can provide both accurate and precise results. In contrast, 1,5,5-trimethylhydantoin (1,5,5-TH) used as the non-isotopic IS for creatinine may cause a bias of over 13% in SI method and even worse when the MSI technique was used. Another compound, 2-methyl-3-indoleacetic acid (2-MIAA), was determined not suitable for MSI analysis of 5-HIAA due to endogenous interferences despite its acceptable performance in conventional methods of analysis. |
URL | https://onlinelibrary.wiley.com/doi/abs/10.1002/elps.201900456 |
DOI | 10.1002/elps.201900456 |
