Our Department

Faculty Directory Header Image
  1. Our Department
  2. History
  3. Lawrence McIntosh

Lawrence McIntosh

Profile

Research and Teaching Interests

Our laboratory uses NMR spectroscopy and related biophysical methods to characterize the structure and dynamics of proteins involved in signal transduction and the regulation of gene expression and in the synthesis/degradation of carbohydrates.

 

Please visit our lab website for more details:  http://otter.biochem.ubc.ca

Contact

Real name: 
Lawrence McIntosh
Email: 
Office Room Number(s): 
Life Sciences Institute 4.551
Office Phone Number: 
604-822-3341
Fax Number: 
604-822-2847

Curriculum Vitae

B.Sc., University of Calgary (1982),

Ph.D., University of Oregon (F.W. Dahlquist, 1990);

Helen Hay Whitney Postdoctoral Fellow, Whitehead Institute, MIT (P.S. Kim, 1991-1992);

Alexander von Humbolt Fellow (1999);

CIHR Scientist (2000);

Publications

2013

Kukic, P. ; Farrell, D. ; McIntosh, L. P. ; Garcia-Moreno, B. E. ; Jensen, K. Steen; Toleikis, Z. ; Teilum, K. ; Nielsen, J. Erik Protein Dielectric Constants Determined From Nmr Chemical Shift Perturbations. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2013, 135, 16968-16976.
Stevens, C. M. ; Okon, M. ; McIntosh, L. P. ; Paetzel, M. H-1, C-13 And N-15 Resonance Assignments And Peptide Binding Site Chemical Shift Perturbation Mapping For The Escherichia Coli Redox Enzyme Chaperone Dmsd. BIOMOLECULAR NMR ASSIGNMENTS 2013, 7, 193-197.
Eklof, J. M. ; Shojania, S. ; Okon, M. ; McIntosh, L. P. ; Brumer, H. Structure-Function Analysis Of A Broad Specificity Populus Trichocarpa Endo-Beta-Glucanase Reveals An Evolutionary Link Between Bacterial Licheninases And Plant Xth Gene Products. JOURNAL OF BIOLOGICAL CHEMISTRY 2013, 288, 15786-15799.
Ludwiczek, M. L. ; D'Angelo, I. ; Yalloway, G. N. ; Brockerman, J. A. ; Okon, M. ; Nielsen, J. E. ; Strynadka, N. C. J. ; Withers, S. G. ; McIntosh, L. P. Strategies For Modulating The Ph-Dependent Activity Of A Family 11 Glycoside Hydrolase. BIOCHEMISTRY 2013, 52, 3138-3156.
Chan, P. H. W. ; Cheung, A. H. ; Okon, M. ; Chen, H. - M. ; Withers, S. G. ; McIntosh, L. P. Investigating The Structural Dynamics Of Alpha-1,4-Galactosyltransferase C From Neisseria Meningitidis By Nuclear Magnetic Resonance Spectroscopy. BIOCHEMISTRY 2013, 52, 320-332.

2012

, ; De, S. ; Okon, M. ; Green, S. M. ; Bhachech, N. ; Graves, B. J. ; McIntosh, L. P. Autoinhibition Of Etv6 (Tel) Dna Binding: Appended Helices Sterically Block The Ets Domain. JOURNAL OF MOLECULAR BIOLOGY 2012, 421, 67-84.
Prehna, G. ; Zhang, G. ; Gong, X. ; Duszyk, M. ; Okon, M. ; McIntosh, L. P. ; Weiner, J. H. ; Strynadka, N. C. J. A Protein Export Pathway Involving Escherichia Coil Porins. STRUCTURE 2012, 20, 1154-1166.
De, S. ; McIntosh, L. P. Putting A Stop To L,d-Transpeptidases. STRUCTURE 2012, 20, 753-754.
Giovinazzi, S. ; Lindsay, C. R. ; Morozov, V. M. ; Escobar-Cabrera, E. ; Summers, M. K. ; Han, H. S. ; McIntosh, L. P. ; Ishov, A. M. Regulation Of Mitosis And Taxane Response By Daxx And Rassf1. ONCOGENE 2012, 31, 13-26.
Lau, D. K. W. ; Okon, M. ; McIntosh, L. P. The Pnt Domain From Drosophila Pointed-P2 Contains A Dynamic N-Terminal Helix Preceded By A Disordered Phosphoacceptor Sequence. PROTEIN SCIENCE 2012, 21, 1716-1725.
Chan, P. H. W. ; Weissbach, S. ; Okon, M. ; Withers, S. G. ; McIntosh, L. P. Nuclear Magnetic Resonance Spectral Assignments Of Alpha-1,4-Galactosyltransferase Lgtc From Neisseria Meningitidis: Substrate Binding And Multiple Conformational States. BIOCHEMISTRY 2012, 51, 8278-8292.
Prehna, G. ; Li, Y. ; Stoynov, N. ; Okon, M. ; Vuckovic, M. ; McIntosh, L. P. ; Foster, L. J. ; B. Finlay, B. ; Strynadka, N. C. J. The Zinc Regulated Antivirulence Pathway Of Salmonella Is A Multiprotein Immunoglobulin Adhesion System. JOURNAL OF BIOLOGICAL CHEMISTRY 2012, 287, 32324-32337.

2011

Baturin, S. J. ; Okon, M. ; McIntosh, L. P. Structure, Dynamics, And Ionization Equilibria Of The Tyrosine Residues In Bacillus Circulans Xylanase. JOURNAL OF BIOMOLECULAR NMR 2011, 51, 379-394.
McIntosh, L. P. ; Naito, D. ; Baturin, S. J. ; Okon, M. ; Joshi, M. D. ; Nielsen, J. E. Dissecting Electrostatic Interactions In Bacillus Circulans Xylanase Through Nmr-Monitored Ph Titrations. JOURNAL OF BIOMOLECULAR NMR 2011, 51, 5-19.
Escobar-Cabrera, E. ; Okon, M. ; Lau, D. K. W. ; Dart, C. F. ; Bonvin, A. M. J. J. ; McIntosh, L. P. Characterizing The N- And C-Terminal Small Ubiquitin-Like Modifier (Sumo)-Interacting Motifs Of The Scaffold Protein Daxx. JOURNAL OF BIOLOGICAL CHEMISTRY 2011, 286, 19816-19829.
Webb, H. ; Tynan-Connolly, B. Mary; Lee, G. M. ; Farrell, D. ; O'Meara, F. ; Sondergaard, C. R. ; Teilum, K. ; Hewage, C. ; McIntosh, L. P. ; Nielsen, J. Erik Remeasuring Hewl Pk(A) Values By Nmr Spectroscopy: Methods, Analysis, Accuracy, And Implications For Theoretical Pk(A), Calculations. PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS 2011, 79, 685-702.
Kim, K. H. ; Kang, H. - S. ; Okon, M. ; Escobar-Cabrera, E. ; McIntosh, L. P. ; Paetzel, M. Structural Characterization Of Escherichia Coli Bame, A Lipoprotein Component Of The Beta-Barrel Assembly Machinery Complex. BIOCHEMISTRY 2011, 50, 1081-1090.
Hollenhorst, P. C. ; McIntosh, L. P. ; Graves, B. J. Genomic And Biochemical Insights Into The Specificity Of Ets Transcription Factors. In ANNUAL REVIEW OF BIOCHEMISTRY, VOL 80; Kornberg, R. D. ; Raetz, C. R. H. ; Rothman, J. E. ; Thorner, J. W. ; ANNUAL REVIEW OF BIOCHEMISTRY, VOL 80; 2011; Vol. 80, pp. 437-471.

2010

Reitinger, S. ; Yu, Y. ; Wicki, J. ; Ludwiczek, M. ; D’Angelo, I. ; Baturin, S. ; Okon, M. ; Strynadka, N. C. J. ; Lutz, S. ; Withers, S. G. ; McIntosh, L. P. Circular Permutation Of Bacillus Circulans Xylanase: A Kinetic And Structural Study. Biochemistry 2010, 49, 2464 - 2474.
Escobar-Cabrera, E. ; Lau, D. K. W. ; Giovinazzi, S. ; Ishov, A. M. ; McIntosh, L. P. Structural Characterization Of The Daxx N-Terminal Helical Bundle Domain And Its Complex With Rassf1C. STRUCTURE 2010, 18, 1642-1653.
Nelson, M. L. ; Kang, H. - S. ; Lee, G. M. ; Blaszczak, A. G. ; Lau, D. K. W. ; McIntosh, L. P. ; Graves, B. J. Ras Signaling Requires Dynamic Properties Of Ets1 For Phosphorylation-Enhanced Binding To Coactivator Cbp. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2010, 107, 10026-10031.
Austin, P. ; Heller, M. ; Williams, D. E. ; McIntosh, L. P. ; A. Vogl, W. ; Foster, L. J. ; Andersen, R. J. ; Roberge, M. ; Roskelley, C. D. Release Of Membrane-Bound Vesicles And Inhibition Of Tumor Cell Adhesion By The Peptide Neopetrosiamide A. PLOS ONE 2010, 5, e10836.
Reitinger, S. ; Yu, Y. ; Wicki, J. ; Ludwiczek, M. ; D'Angelo, I. ; Baturin, S. ; Okon, M. ; Strynadka, N. C. J. ; Lutz, S. ; Withers, S. G. ; McIntosh, L. P. Circular Permutation Of Bacillus Circulans Xylanase: A Kinetic And Structural Study. BIOCHEMISTRY 2010, 49, 2464-2474.
Farrell, D. ; Miranda, E. Sa; Webb, H. ; Georgi, N. ; Crowley, P. B. ; McIntosh, L. P. ; Nielsen, J. Erik Titration\_Db: Storage And Analysis Of Nmr-Monitored Protein Ph Titration Curves. PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS 2010, 78, 843-857.

2009

McIntosh, L. P. ; Kang, H. S. ; Okon, M. ; Nelson, M. L. ; Graves, B. J. ; Brutscher, B. Detection And Assignment Of Phosphoserine And Phosphothreonine Residues By C-13-P-31 Spin-Echo Difference Nmr Spectroscopy. Journal of Biomolecular Nmr 2009, 43, 31-37.
Chan, P. H. W. ; Lairson, L. L. ; Lee, H. J. ; Wakarchuk, W. W. ; Strynadka, N. C. J. ; Withers, S. G. ; McIntosh, L. P. Nmr Spectroscopic Characterization Of The Sialyltransferase Cstii From Campylobacter Jejuni: Histidine 188 Is The General Base. Biochemistry 2009, 48, 11220-11230.

2008

Wilke, M. S. ; Heller, M. ; Creagh, A. L. ; Haynes, C. A. ; McIntosh, L. P. ; Poole, K. ; Strynadka, N. C. J. The Crystal Structure Of Mexr From Pseudomonas Aeruginosa In Complex With Its Antirepressor Armr. Proceedings of the National Academy of Sciences of the United States of America 2008, 105, 14832-14837.
Lee, G. M. ; Pufall, M. A. ; Meeker, C. A. ; Kang, H. S. ; Graves, B. J. ; McIntosh, L. P. The Affinity Of Ets-1 For Dna Is Modulated By Phosphorylation Through Transient Interactions Of An Unstructured Region. Journal of Molecular Biology 2008, 382, 1014-1030.
Kang, H. S. ; Nelson, M. L. ; Mackereth, C. D. ; Scharpf, M. ; Graves, B. J. ; McIntosh, L. P. Identification And Structural Characterization Of A Cbp/p300-Binding Domain From The Ets Family Transcription Factor Gabp Alpha. Journal of Molecular Biology 2008, 377, 636-646.
Feldman, A. R. ; Shapova, Y. A. ; Wu, S. S. T. ; Oliver, D. C. ; Heller, M. ; McIntosh, L. P. ; Scott, J. K. ; Paetzel, M. Phage Display And Crystallographic Analysis Reveals Potential Substrate/binding Site Interactions In The Protein Secretion Chaperone Csaa From Agrobacterium Tumefaciens. Journal of Molecular Biology 2008, 379, 457-470.
Sondergaard, C. R. ; McIntosh, L. P. ; Pollastri, G. ; Nielsen, J. E. Determination Of Electrostatic Interaction Energies And Protonation State Populations In Enzyme Active Sites. Journal of Molecular Biology 2008, 376, 269-287.

2007

Poon, D. K. Y. ; Ludwiczek, M. L. ; Schubert, M. ; Kwan, E. M. ; Withers, S. G. ; McIntosh, L. P. Nmr Spectroscopic Characterization Of A Beta-(1,4)-Glycosidase Along Its Reaction Pathway: Stabilization Upon Formation Of The Glycosyl-Enzyme Intermediate. Biochemistry 2007, 46, 1759-1770.
Lakowski, T. M. ; Lee, G. M. ; Okon, M. ; Reid, R. E. ; McIntosh, L. P. Calcium-Induced Folding Of A Fragment Of Calmodulin Composed Of Ef-Hands 2 And 3. Protein Science 2007, 16, 1119-1132.
Schubert, M. ; Poon, D. K. Y. ; Wicki, J. ; Tarling, C. A. ; Kwan, E. M. ; Nielsen, J. E. ; Withers, S. G. ; McIntosh, L. P. Probing Electrostatic Interactions Along The Reaction Pathway Of A Glycoside Hydrolase: Histidine Characterization By Nmr Spectroscopy. Biochemistry 2007, 46, 7383-7395.
Lakowski, T. M. ; Lee, G. M. ; Lelj-Garolla, B. ; Okon, M. ; Reid, R. E. ; McIntosh, L. P. Peptide Binding By A Fragment Of Calmodulin Composed Of Ef-Hands 2 And 3. Biochemistry 2007, 46, 8525-8536.
Poon, D. K. Y. ; Withers, S. G. ; McIntosh, L. P. Direct Demonstration Of The Flexibility Of The Glycosylated Proline-Threonine Linker In The Cellulomonas Fimi Xylanase Cex Through Nmr Spectroscopic Analysis. Journal of Biological Chemistry 2007, 282, 2091-2100.

2006

Macauley, M. S. ; Errington, W. J. ; Scharpf, M. ; Mackereth, C. D. ; Blaszczak, A. G. ; Graves, B. J. ; McIntosh, L. P. Beads-On-A-String, Characterization Of Ets-1 Sumoylated Within Its Flexible N-Terminal Sequence. Journal of Biological Chemistry 2006, 281, 4164-4172.
Kim, Y. W. ; Lovering, A. L. ; Chen, H. M. ; Kantner, T. ; McIntosh, L. P. ; Strynadka, N. C. J. ; Withers, S. G. Expanding The Thioglycoligase Strategy To The Synthesis Of Alpha-Linked Thioglycosides Allows Structural Investigation Of The Parent Enzyme/substrate Complex. Journal of the American Chemical Society 2006, 128, 2202-2203.
Poon, D. K. Y. ; Schubert, M. ; Au, J. ; Okon, M. ; Withers, S. G. ; McIntosh, L. P. Unambiguous Determination Of The Ionization State Of A Glycoside Hydrolase Active Site Lysine By H-1-N-15 Heteronuclear Correlation Spectroscopy. Journal of the American Chemical Society 2006, 128, 15388-15389.
Kim, Y. W. ; Fox, D. T. ; Hekmat, O. ; Kantner, T. ; McIntosh, L. P. ; Warren, R. A. J. ; Withers, S. G. Glycosynthase-Based Synthesis Of Xylo-Oligosaccharides Using An Engineered Retaining Xylanase From Cellulomonas Fimi. Organic & Biomolecular Chemistry 2006, 4, 2025-2032.

2005

Escobar-Cabrera, E. ; Venkatesan, M. ; Desautels, M. ; Hemmingsen, S. M. ; McIntosh, L. P. Dissecting The Domain Structure Of Cdc4P, A Myosin Essential Light Chain Involved In Schizosaccharomyces Pombe Cytokinesis. Biochemistry 2005, 44, 12136-12148.
Pufall, M. A. ; Lee, G. M. ; Nelson, M. L. ; Kang, H. S. ; Velyvis, A. ; Kay, L. E. ; McIntosh, L. P. ; Graves, B. J. Variable Control Of Ets-1 Dna Binding By Multiple Phosphates In An Unstructured Region. Science 2005, 309, 142-145.
Lee, G. M. ; Donaldson, L. W. ; Pufall, M. A. ; Kang, H. S. ; Pot, I. ; Graves, B. J. ; McIntosh, L. P. The Structural And Dynamic Basis Of Ets-1 Dna Binding Autoinhibition. Journal of Biological Chemistry 2005, 280, 7088-7099.
Okon, M. ; Pfuetzner, R. ; Vuckovic, M. ; Little, J. W. ; Strynadka, N. C. J. ; McIntosh, L. P. Letter To The Editor: Backbone Chemical Shift Assignments Of The Lexa Catalytic Domain In Its Active Conformation. Journal of Biomolecular Nmr 2005, 31, 371-372.

2004

Mackereth, C. D. ; Scharpf, M. ; Gentile, L. N. ; Macintosh, S. E. ; Slupsky, C. M. ; McIntosh, L. P. Diversity In Structure And Function Of The Ets Family Pnt Domains. Journal of Molecular Biology 2004, 342, 1249-1264.
Macauley, M. S. ; Errington, W. J. ; Okon, M. ; Scharpf, M. ; Mackereth, C. D. ; Schulman, B. A. ; McIntosh, L. P. Structural And Dynamic Independence Of Isopeptide-Linked Rangap1 And Sumo-1. Journal of Biological Chemistry 2004, 279, 49131-49137.
Erbel, P. J. A. ; Seidel, R. ; Macintosh, S. E. ; Gentile, L. N. ; Amor, J. C. ; Kahn, R. A. ; Prestegard, J. H. ; McIntosh, L. P. ; Gardner, K. H. Cyclic Enterobacterial Common Antigen: Potential Contaminant Of Bacterially Expressed Protein Preparations. Journal of Biomolecular Nmr 2004, 29, 199-204.
Tognon, C. E. ; Mackereth, C. D. ; Somasiri, A. M. ; McIntosh, L. P. ; Sorensen, P. H. B. Mutations In The Sam Domain Of The Etv6-Ntrk3 Chimeric Tyrosine Kinase Block Polymerization And Transformation Activity. Molecular and Cellular Biology 2004, 24, 4636-4650.
Schubert, M. ; Edge, R. E. ; Lario, P. ; Cook, M. A. ; Strynadka, N. C. J. ; Mackie, G. A. ; McIntosh, L. P. Structural Characterization Of The Rnase E S1 Domain And Identification Of Its Oligonucleotide-Binding And Dimerization Interfaces. Journal of Molecular Biology 2004, 341, 37-54.

2003

Poon, D. K. Y. ; Webster, P. ; Withers, S. G. ; McIntosh, L. P. Characterizing The Ph-Dependent Stability And Catalytic Mechanism Of The Family 11 Xylanase From The Alkalophilic Bacillus Agaradhaerens. Carbohydrate Research 2003, 338, 415-421.

2002

Ramachander, R. ; Kim, C. A. ; Phillips, M. L. ; Mackereth, C. D. ; Thanos, C. D. ; McIntosh, L. P. ; Bowie, J. U. Oligomerization-Dependent Association Of The Sam Domains From Schizosaccharomyces Pombe Byr2 And Ste4. Journal of Biological Chemistry 2002, 277, 39585-39593.
Wang, H. ; McIntosh, L. P. ; Graves, B. J. Inhibitory Module Of Ets-1 Allosterically Regulates Dna Binding Through A Dipole-Facilitated Phosphate Contact. Journal of Biological Chemistry 2002, 277, 2225-2233.
Yee, A. ; Chang, X. Q. ; Pineda-Lucena, A. ; Wu, B. ; Semesi, A. ; Le, B. ; Ramelot, T. ; Lee, G. M. ; Bhattacharyya, S. ; Gutierrez, P. ; Denisov, A. ; Lee, C. H. ; Cort, J. R. ; Kozlov, G. ; Liao, J. ; Finak, G. ; Chen, L. ; Wishart, D. ; Lee, W. ; McIntosh, L. P. ; Gehring, K. ; Kennedy, M. A. ; Edwards, A. M. ; Arrowsmith, C. H. An Nmr Approach To Structural Proteomics. Proceedings of the National Academy of Sciences of the United States of America 2002, 99, 1825-1830.
Griswold, I. J. ; Zhou, H. J. ; Matison, M. ; Swanson, R. V. ; McIntosh, L. P. ; Simon, M. I. ; Dahlquist, F. W. The Solution Structure And Interactions Of Chew From Thermotoga Maritima. Nature Structural Biology 2002, 9, 121-125.
Scharpf, M. ; Connelly, G. P. ; Lee, G. M. ; Boraston, A. B. ; Warren, R. A. J. ; McIntosh, L. P. Site-Specific Characterization Of The Association Of Xylooligosaccharides With The Cbm13 Lectin-Like Xylan Binding Domain From Streptomyces Lividans Xylanase 10A By Nmr Spectroscopy. Biochemistry 2002, 41, 4255-4263.
Mackereth, C. D. ; Scharpf, M. ; Gentile, L. N. ; McIntosh, L. P. Letter To The Editor: Chemical Shift And Secondary Structure Conservation Of The Pnt/sam Domains From The Ets Family Of Transcription Factors. Journal of Biomolecular Nmr 2002, 24, 71-72.

2001

Lee, G. M. ; Edwards, A. M. ; Arrowsmith, C. H. ; McIntosh, L. P. Letter To The Editor: Nmr-Based Structure Of The Conserved Protein Mth865 From The Archaeon Methanobacterium Thermoautotrophicum. Journal of Biomolecular Nmr 2001, 21, 63-66.
Desautels, M. ; Haese, J. P. Den; Slupsky, C. M. ; McIntosh, L. P. ; Hemmingsen, S. M. Cdc4P, A Contractile Ring Protein Essential For Cytokinesis In Schizosaccharomyces Pombe, Interacts With A Phosphatidylinositol 4-Kinase. Journal of Biological Chemistry 2001, 276, 5932-5942.
Slupsky, C. M. ; Desautels, M. ; Huebert, T. ; Zhao, R. H. ; Hemmingsen, S. M. ; McIntosh, L. P. Structure Of Cdc4P, A Contractile Ring Protein Essential For Cytokinesis In Schizosaccharomyces Pombe. Journal of Biological Chemistry 2001, 276, 5943-5951.
Joshi, M. D. ; Sidhu, G. ; Nielsen, J. E. ; Brayer, G. D. ; Withers, S. G. ; McIntosh, L. P. Dissecting The Electrostatic Interactions And Ph-Dependent Activity Of A Family 11 Glycosidase. Biochemistry 2001, 40, 10115-10139.
Brun, E. ; Brumer, H. ; Mackenzie, L. F. ; Withers, S. G. ; McIntosh, L. P. Letter To The Editor: Assignment Of Selectively C-13-Labeled Cellopentaose Synthesized Using An Engineered Glycosynthase. Journal of Biomolecular Nmr 2001, 21, 67-68.

2000

Christendat, D. ; Yee, A. ; Dharamsi, A. ; Kluger, Y. ; Savchenko, A. ; Cort, J. R. ; Booth, V. ; Mackereth, C. D. ; Saridakis, V. ; Ekiel, I. ; Kozlov, G. ; Maxwell, K. L. ; Wu, N. ; McIntosh, L. P. ; Gehring, K. ; Kennedy, M. A. ; Davidson, A. R. ; Pai, E. F. ; Gerstein, M. ; Edwards, A. M. ; Arrowsmith, C. H. Structural Proteomics Of An Archaeon. Nature Structural Biology 2000, 7, 903-909.
Joshi, M. D. ; Sidhu, G. ; Pot, I. ; Brayer, G. D. ; Withers, S. G. ; McIntosh, L. P. Hydrogen Bonding And Catalysis: A Novel Explanation For How A Single Amino Acid Substitution Can Change The Ph Optimum Of A Glycosidase. Journal of Molecular Biology 2000, 299, 255-279.
Connelly, G. P. ; Withers, S. G. ; McIntosh, L. P. Analysis Of The Dynamic Properties Of Bacillus Circulans Xylanase Upon Formation Of A Covalent Glycosyl-Enzyme Intermediate. Protein Science 2000, 9, 512-524.
Brun, E. ; Johnson, P. E. ; Creagh, A. L. ; Tomme, P. ; Webster, P. ; Haynes, C. A. ; McIntosh, L. P. Structure And Binding Specificity Of The Second N-Terminal Cellulose-Binding Domain From Cellulomonas Fimi Endoglucanase C. Biochemistry 2000, 39, 2445-2458.
Mackereth, C. D. ; Arrowsmith, C. H. ; Edwards, A. M. ; McIntosh, L. P. Zinc-Bundle Structure Of The Essential Rna Polymerase Subunit Rpb10 From Methanobacterium Thermoautotrophicum. Proceedings of the National Academy of Sciences of the United States of America 2000, 97, 6316-6321.
Kormos, J. ; Johnson, P. E. ; Brun, E. ; Tomme, P. ; McIntosh, L. P. ; Haynes, C. A. ; Kilburn, D. G. Binding Site Analysis Of Cellulose Binding Domain Cbdn1 From Endoglucanse C Of Cellulomonas Fimi By Site-Directed Mutagenesis. Biochemistry 2000, 39, 8844-8852.

1999

Wheat, W. ; Fitzsimmons, D. ; Lennox, H. ; Krautkramer, S. R. ; Gentile, L. N. ; McIntosh, L. P. ; Hagman, J. The Highly Conserved Beta-Hairpin Of The Paired Dna-Binding Domain Is Required For Assembly Of Pax-Ets Ternary Complexes. Molecular and Cellular Biology 1999, 19, 2231-2241.
Johnson, P. E. ; Brun, E. ; Mackenzie, L. F. ; Withers, S. G. ; McIntosh, L. P. The Cellulose-Binding Domains From Cellulomonas Fimi Beta-1,4-Glucanase Cenc Bind Nitroxide Spin-Labeled Cellooligosaccharides In Multiple Orientations. Journal of Molecular Biology 1999, 287, 609-625.
Sidhu, G. ; Withers, S. G. ; Nguyen, N. T. ; McIntosh, L. P. ; Ziser, L. ; Brayer, G. D. Sugar Ring Distortion In The Glycosyl-Enzyme Intermediate Of A Family G/11 Xylanase. Biochemistry 1999, 38, 5346-5354.

1998

Birsan, C. ; Johnson, P. ; Joshi, M. ; MacLeod, A. ; McIntosh, L. ; Monem, V. ; Nitz, M. ; Rose, D. R. ; Tull, D. ; Wakarchuck, W. W. ; Wang, Q. ; Warren, R. A. J. ; White, A. ; Withers, S. G. Mechanisms Of Cellulases And Xylanases. Biochemical Society Transactions 1998, 26, 156-160.
Johnson, P. E. ; Creagh, A. L. ; Brun, E. ; Joe, K. ; Tomme, P. ; Haynes, C. A. ; McIntosh, L. P. Calcium Binding By The N-Terminal Cellulose-Binding Domain From Cellulomonas Fimi Beta-1,4-Glucanase Cenc. Biochemistry 1998, 37, 12772-12781.
Slupsky, C. M. ; Gentile, L. N. ; Donaldson, L. W. ; Mackereth, C. D. ; Seidel, J. J. ; Graves, B. J. ; McIntosh, L. P. Structure Of The Ets-1 Pointed Domain And Mitogen-Activated Protein Kinase Phosphorylation Site. Proceedings of the National Academy of Sciences of the United States of America 1998, 95, 12129-12134.
Creagh, A. L. ; Koska, J. ; Johnson, P. E. ; Tomme, P. ; Joshi, M. D. ; McIntosh, L. P. ; Kilburn, D. G. ; Haynes, C. A. Stability And Oligosaccharide Binding Of The N1 Cellulose-Binding Domain Of Cellulomonas Fimi Endoglucanase Cenc. Biochemistry 1998, 37, 3529-3537.
Connelly, G. P. ; McIntosh, L. P. Characterization Of A Buried Neutral Histidine In Bacillus Circulans Xylanase: Internal Dynamics And Interaction With A Bound Water Molecule. Biochemistry 1998, 37, 1810-1818.
Slupsky, C. M. ; Gentile, L. N. ; McIntosh, L. P. Assigning The Nmr Spectra Of Aromatic Amino Acids In Proteins: Analysis Of Two Ets Pointed Domains. Biochemistry and Cell Biology-Biochimie Et Biologie Cellulaire 1998, 76, 379-390.

1997

Muhandiram, D. R. ; Johnson, P. E. ; Yang, D. W. ; Zhang, O. W. ; McIntosh, L. P. ; Kay, L. E. Specific N-15,nh Correlations For Residues In N-15,c-13 And Fractionally Deuterated Proteins That Immediately Follow Methyl-Containing Amino Acids. Journal of Biomolecular Nmr 1997, 10, 283-288.
Joshi, M. D. ; Hedberg, A. ; McIntosh, L. P. Complete Measurement Of The Pk(A) Values Of The Carboxyl And Imidazole Groups In Bacillus Circulans Xylanase. Protein Science 1997, 6, 2667-2670.
Feher, V. A. ; Zapf, J. W. ; Hoch, J. A. ; Whiteley, J. M. ; McIntosh, L. P. ; Rance, M. ; Skelton, N. J. ; Dahlquist, F. W. ; Cavanah, J. High-Resolution Nmr Structure And Backbone Dynamics Of The Bacillus Subtilis Response Regulator, Spoof: Implications For Phosphorylation And Molecular Recognition. Biochemistry 1997, 36, 10015-10025.
McIntosh, L. P. ; Brun, E. ; Kay, L. E. Stereospecific Assignment Of The Nh2 Resonances From The Primary Amides Of Asparagine And Glutamine Side Chains In Isotopically Labeled Proteins. Journal of Biomolecular Nmr 1997, 9, 306-312.

1996

Plesniak, L. A. ; Wakarchuk, W. W. ; McIntosh, L. P. Secondary Structure And Nmr Assignments Of Bacillus Circulans Xylanase. Protein Science 1996, 5, 1118-1135.
Donaldson, L. W. ; Petersen, J. M. ; Graves, B. J. ; McIntosh, L. P. Solution Structure Of The Ets Domain From Murine Ets-1: A Winged Helix-Turn-Helix Dna Binding Motif. Embo Journal 1996, 15, 125-134.
Skalicky, J. J. ; Donaldson, L. W. ; Petersen, J. M. ; Graves, B. J. ; McIntosh, L. P. Structural Coupling Of The Inhibitory Regions Flanking The Ets Domain Of Murine Ets-1. Protein Science 1996, 5, 296-309.
Tomme, P. ; Gilkes, N. R. ; Guarna, M. M. ; Haynes, C. A. ; Hasenwinkle, D. ; Jervis, E. ; Johnson, P. ; McIntosh, L. ; Warren, R. A. J. ; Kilburn, D. G. Cellulose-Binding Domains - Versatile Affinity Tags For Inexpensive Large-Scale Purification, Concentration, And Immobilization Of Fusion Proteins. In Enzyme Engineering Xiii; Dordick, J. S. ; Russell, A. J. ; Enzyme Engineering Xiii; New York Acad Sciences: New York, 1996; Vol. 799, pp. 418-424.
McIntosh, L. P. ; Hand, G. ; Johnson, P. E. ; Joshi, M. D. ; Korner, M. ; Plesniak, L. A. ; Ziser, L. ; Wakarchuk, W. W. ; Withers, S. G. The Pk(A) Of The General Acid/base Carboxyl Group Of A Glycosidase Cycles During Catalysis: A C-13-Nmr Study Of Bacillus Circuluns Xylanase. Biochemistry 1996, 35, 9958-9966.
Plesniak, L. A. ; Connelly, G. P. ; Wakarchuk, W. W. ; McIntosh, L. P. Characterization Of A Buried Neutral Histidine Residue In Bacillus Circulans Xylanase: Nmr Assignments, Ph Titration, And Hydrogen Exchange. Protein Science 1996, 5, 2319-2328.
Johnson, P. E. ; Tomme, P. ; Joshi, M. D. ; McIntosh, L. P. Interaction Of Soluble Cellooligosaccharides With The N-Terminal Cellulose-Binding Domain Of Cellulomonas Fimi Cenc .2. Nmr And Ultraviolet Absorption Spectroscopy. Biochemistry 1996, 35, 13895-13906.
Bray, M. R. ; Johnson, P. E. ; Gilkes, N. R. ; McIntosh, L. P. ; Kilburn, D. G. ; Warren, R. A. J. Probing The Role Of Tryptophan Residues In A Cellulose-Binding Domain By Chemical Modification. Protein Science 1996, 5, 2311-2318.
Johnson, P. E. ; Joshi, M. D. ; Tomme, P. ; Kilburn, D. G. ; McIntosh, L. P. Structure Of The N-Terminal Cellulose-Binding Domain Of Cellulomonas Fimi Cenc Determined By Nuclear Magnetic Resonance Spectroscopy. Biochemistry 1996, 35, 14381-14394.

1995

McIntosh, L. ; Plesniak, L. ; Johnson, P. ; Withers, S. G. ; Wakarchuk, W. The Structure And Enzymatic Mechanism Of Bacillus-Circulans Xylanase. Journal of Cellular Biochemistry 1995, 30-30.
Petersen, J. M. ; Skalicky, J. J. ; Donaldson, L. W. ; McIntosh, L. P. ; Alber, T. ; Graves, B. J. Modulation Of Transcription Factor Ets-1 Dna-Binding - Dna-Induced Unfolding Of An Alpha-Helix. Science 1995, 269, 1866-1869.
Wilkens, S. ; Dahlquist, F. W. ; McIntosh, L. P. ; Donaldson, L. W. ; Capaldi, R. A. Structural Features Of The Epsilon-Subunit Of The Escherichia-Coli Atp Synthase Determined By Nmr-Spectroscopy. Nature Structural Biology 1995, 2, 961-967.
Johnson, P. E. ; Tomme, P. ; McIntosh, L. P. Structural Studies Of The N1 Cellulose-Binding Domain From Cellulomonas-Fimi Endoglucanse-C. Journal of Cellular Biochemistry 1995, 46-46.

1994

Donaldson, L. W. ; Petersen, J. M. ; Graves, B. J. ; McIntosh, L. P. Secondary Structure Of The Ets Domain Places Murine Ets-1 In The Superfamily Of Winged Helix-Turn-Helix Dna-Binding Proteins. Biochemistry 1994, 33, 13509-13516.

Links

Lab website